This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1kf3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1kf3.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1kf3.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1kf3| PDB=1kf3 | SCENE= }}
{{STRUCTURE_1kf3| PDB=1kf3 | SCENE= }}
-
'''Atomic Resolution Structure of RNase A at pH 5.9'''
+
===Atomic Resolution Structure of RNase A at pH 5.9===
-
==Overview==
+
<!--
-
The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11856829}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11856829 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11856829}}
==About this Structure==
==About this Structure==
Line 34: Line 38:
[[Category: Soaking]]
[[Category: Soaking]]
[[Category: Titration]]
[[Category: Titration]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:39:57 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:14:51 2008''

Revision as of 07:14, 2 July 2008

Image:1kf3.png

Template:STRUCTURE 1kf3

Atomic Resolution Structure of RNase A at pH 5.9

Template:ABSTRACT PUBMED 11856829

About this Structure

1KF3 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:11856829

Page seeded by OCA on Wed Jul 2 10:14:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kf3"
Personal tools