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1kft

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[[Image:1kft.gif|left|200px]]
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{{STRUCTURE_1kft| PDB=1kft | SCENE= }}
{{STRUCTURE_1kft| PDB=1kft | SCENE= }}
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'''Solution Structure of the C-Terminal domain of UvrC from E-coli'''
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===Solution Structure of the C-Terminal domain of UvrC from E-coli===
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==Overview==
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The C-terminal domain of the UvrC protein (UvrC CTD) is essential for 5' incision in the prokaryotic nucleotide excision repair process. We have determined the three-dimensional structure of the UvrC CTD using heteronuclear NMR techniques. The structure shows two helix-hairpin-helix (HhH) motifs connected by a small connector helix. The UvrC CTD is shown to mediate structure-specific DNA binding. The domain binds to a single-stranded-double-stranded junction DNA, with a strong specificity towards looped duplex DNA that contains at least six unpaired bases per loop ("bubble DNA"). Using chemical shift perturbation experiments, the DNA-binding surface is mapped to the first hairpin region encompassing the conserved glycine-valine-glycine residues followed by lysine-arginine-arginine, a positively charged surface patch and the second hairpin region consisting of glycine-isoleucine-serine. A model for the protein-DNA complex is proposed that accounts for this specificity.
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The line below this paragraph, {{ABSTRACT_PUBMED_12426397}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12426397 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12426397}}
==About this Structure==
==About this Structure==
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1KFT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFT OCA].
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1KFT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFT OCA].
==Reference==
==Reference==
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[[Category: Helix-hairpin-helix]]
[[Category: Helix-hairpin-helix]]
[[Category: Hhh domain]]
[[Category: Hhh domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:41:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:16:52 2008''

Revision as of 07:16, 2 July 2008

Image:1kft.png

Template:STRUCTURE 1kft

Solution Structure of the C-Terminal domain of UvrC from E-coli

Template:ABSTRACT PUBMED 12426397

About this Structure

1KFT is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli., Singh S, Folkers GE, Bonvin AM, Boelens R, Wechselberger R, Niztayev A, Kaptein R, EMBO J. 2002 Nov 15;21(22):6257-66. PMID:12426397

Page seeded by OCA on Wed Jul 2 10:16:52 2008

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