1jm4
From Proteopedia
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| - | [[Image:1jm4.gif|left|200px]]<br /> | + | [[Image:1jm4.gif|left|200px]]<br /><applet load="1jm4" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1jm4" size=" | + | |
caption="1jm4" /> | caption="1jm4" /> | ||
'''NMR Structure of P/CAF Bromodomain in Complex with HIV-1 Tat Peptide'''<br /> | '''NMR Structure of P/CAF Bromodomain in Complex with HIV-1 Tat Peptide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The human immunodeficiency virus type 1 (HIV-1) trans-activator protein | + | The human immunodeficiency virus type 1 (HIV-1) trans-activator protein Tat stimulates transcription of the integrated HIV-1 genome and promotes viral replication in infected cells. Tat transactivation activity is dependent on lysine acetylation and its association with nuclear histone acetyltransferases p300/CBP (CREB binding protein) and p300/CBP-associated factor (PCAF). Here, we show that the bromodomain of PCAF binds specifically to HIV-1 Tat acetylated at lysine 50 and that this interaction competes effectively against HIV-1 TAR RNA binding to the lysine-acetylated Tat. The three-dimensional solution structure of the PCAF bromodomain in complex with a lysine 50-acetylated Tat peptide together with biochemical analyses provides the structural basis for the specificity of this molecular recognition and reveals insights into the differences in ligand selectivity of bromodomains. |
==About this Structure== | ==About this Structure== | ||
| - | 1JM4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1JM4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JM4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Carlson, J | + | [[Category: Carlson, J E.]] |
[[Category: Farooq, A.]] | [[Category: Farooq, A.]] | ||
[[Category: He, Y.]] | [[Category: He, Y.]] | ||
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[[Category: Verdin, E.]] | [[Category: Verdin, E.]] | ||
[[Category: Zeng, L.]] | [[Category: Zeng, L.]] | ||
| - | [[Category: Zhou, M | + | [[Category: Zhou, M M.]] |
[[Category: bromodomain]] | [[Category: bromodomain]] | ||
[[Category: protein-peptide complex]] | [[Category: protein-peptide complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:18 2008'' |
Revision as of 11:24, 21 February 2008
|
NMR Structure of P/CAF Bromodomain in Complex with HIV-1 Tat Peptide
Overview
The human immunodeficiency virus type 1 (HIV-1) trans-activator protein Tat stimulates transcription of the integrated HIV-1 genome and promotes viral replication in infected cells. Tat transactivation activity is dependent on lysine acetylation and its association with nuclear histone acetyltransferases p300/CBP (CREB binding protein) and p300/CBP-associated factor (PCAF). Here, we show that the bromodomain of PCAF binds specifically to HIV-1 Tat acetylated at lysine 50 and that this interaction competes effectively against HIV-1 TAR RNA binding to the lysine-acetylated Tat. The three-dimensional solution structure of the PCAF bromodomain in complex with a lysine 50-acetylated Tat peptide together with biochemical analyses provides the structural basis for the specificity of this molecular recognition and reveals insights into the differences in ligand selectivity of bromodomains.
About this Structure
1JM4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis of lysine-acetylated HIV-1 Tat recognition by PCAF bromodomain., Mujtaba S, He Y, Zeng L, Farooq A, Carlson JE, Ott M, Verdin E, Zhou MM, Mol Cell. 2002 Mar;9(3):575-86. PMID:11931765
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