1khm

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{{STRUCTURE_1khm| PDB=1khm | SCENE= }}
{{STRUCTURE_1khm| PDB=1khm | SCENE= }}
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'''C-TERMINAL KH DOMAIN OF HNRNP K (KH3)'''
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===C-TERMINAL KH DOMAIN OF HNRNP K (KH3)===
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==Overview==
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Among it's many reported functions, heterogeneous nuclear ribonucleoprotein (hnRNP) K is a transcription factor for the c- myc gene, a proto-oncogene critical for the regulation of cell growth and differentiation. We have determined the solution structure of the Gly26--&gt;Arg mutant of the C-terminal K-homology (KH) domain of hnRNP K by NMR spectroscopy. This is the first structure investigation of hnRNP K. Backbone residual dipolar couplings, which provide information that is fundamentally different from the standard NOE-derived distance restraints, were employed to improve structure quality. An independent assessment of structure quality was achieved by comparing the backbone15N T1/T2ratios to the calculated structures. The C-terminal KH module of hnRNP K (KH3) is revealed to be a three-stranded beta-sheet stacked against three alpha-helices, two of which are nearly parallel to the strands of the beta-sheet. The Gly26--&gt;Arg mutation abolishes single-stranded DNA binding without altering the overall fold of the protein. This provides a clue to possible nucleotide binding sites of KH3. It appears unlikely that the solvent-exposed side of the beta-sheet will be the site of protein-nucleic acid complex formation. This is in contrast to the earlier theme for protein-RNA complexes incorporating proteins structurally similar to KH3. We propose that the surface of KH3 that interacts with nucleic acid is comparable to the region of DNA interaction for the double-stranded DNA-binding domain of bovine papillomavirus-1 E2 that has a three-dimensional fold similar to that of KH3.
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{{ABSTRACT_PUBMED_10369774}}
==About this Structure==
==About this Structure==
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1KHM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHM OCA].
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1KHM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHM OCA].
==Reference==
==Reference==
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[[Category: Rna-binding]]
[[Category: Rna-binding]]
[[Category: Three-dimensional structure]]
[[Category: Three-dimensional structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:21:30 2008''

Revision as of 07:21, 2 July 2008

Image:1khm.png

Template:STRUCTURE 1khm

C-TERMINAL KH DOMAIN OF HNRNP K (KH3)

Template:ABSTRACT PUBMED 10369774

About this Structure

1KHM is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

High precision solution structure of the C-terminal KH domain of heterogeneous nuclear ribonucleoprotein K, a c-myc transcription factor., Baber JL, Libutti D, Levens D, Tjandra N, J Mol Biol. 1999 Jun 18;289(4):949-62. PMID:10369774

Page seeded by OCA on Wed Jul 2 10:21:30 2008

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