1kij

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1kij.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1kij.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1kij| PDB=1kij | SCENE= }}
{{STRUCTURE_1kij| PDB=1kij | SCENE= }}
-
'''Crystal structure of the 43K ATPase domain of Thermus thermophilus gyrase B in complex with novobiocin'''
+
===Crystal structure of the 43K ATPase domain of Thermus thermophilus gyrase B in complex with novobiocin===
-
==Overview==
+
<!--
-
DNA gyrase forms an A(2)B(2) tetramer involved in DNA replication, repair, recombination, and transcription in which the B subunit catalyzes ATP hydrolysis. The Thermus thermophilus and Escherichia coli gyrases are homologues and present the same catalytic activity. When compared with that of the E. coli 43K-5'-adenylyl-beta,gamma-imidodiphosphate complex, the crystal structure of Gyrase B 43K ATPase domain in complex with novobiocin, one of the most potent inhibitors of gyrase shows large conformational changes of the subdomains within the dimer. The stabilization of loop 98-118 closing the active site through dimeric contacts and interaction with domain 2 allows to observe novobiocin-protein interactions that could not be seen in the 24K-inhibitor complexes. Furthermore, this loop adopts a position which defines an "open" conformation of the active site in absence of ATP, in contrast with the "closed" conformation adopted upon ATP binding. All together, these results indicate how the subdomains may propagate conformational changes from the active site and provide crucial information for the design of more specific inhibitors.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11850422}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11850422 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11850422}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Gyrase b-coumarin complex]]
[[Category: Gyrase b-coumarin complex]]
[[Category: Topoisomerase]]
[[Category: Topoisomerase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:47:24 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:23:54 2008''

Revision as of 07:23, 2 July 2008

Image:1kij.png

Template:STRUCTURE 1kij

Crystal structure of the 43K ATPase domain of Thermus thermophilus gyrase B in complex with novobiocin

Template:ABSTRACT PUBMED 11850422

About this Structure

1KIJ is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

An open conformation of the Thermus thermophilus gyrase B ATP-binding domain., Lamour V, Hoermann L, Jeltsch JM, Oudet P, Moras D, J Biol Chem. 2002 May 24;277(21):18947-53. Epub 2002 Feb 15. PMID:11850422

Page seeded by OCA on Wed Jul 2 10:23:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kij"
Personal tools