1kio

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[[Image:1kio.jpg|left|200px]]
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[[Image:1kio.png|left|200px]]
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{{STRUCTURE_1kio| PDB=1kio | SCENE= }}
{{STRUCTURE_1kio| PDB=1kio | SCENE= }}
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'''SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]'''
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===SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]===
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==Overview==
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The solution structure of three small serine proteinase inhibitors, two natural and one engineered protein, SGCI (Schistocerca gregaria chymotrypsin inhibitor), SGCI[L30R, K31M] and SGTI (Schistocerca gregaria trypsin inhibitor), were determined by homonuclear NMR-spectroscopy. The molecules exhibit different specificities towards target proteinases, where SGCI is a good chymotrypsin inhibitor, its mutant is a potent trypsin inhibitor, and SGTI inhibits both proteinases weakly. Interestingly, SGTI is a much better inhibitor of insect proteinases than of the mammalian ones used in common assays. All three molecules have a similar fold composed from three antiparallel beta-pleated sheets with three disulfide bridges. The proteinase binding loop has a somewhat distinct geometry in all three peptides. Moreover, the stabilization of the structure is different in SGCI and SGTI. Proton-deuterium exchange experiments are indicative of a highly rigid core in SGTI but not in SGCI. We suggest that the observed structural properties play a significant role in the specificity of these inhibitors.
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(as it appears on PubMed at http://www.pubmed.gov), where 11856311 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11856311}}
==About this Structure==
==About this Structure==
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1KIO is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIO OCA].
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1KIO is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIO OCA].
==Reference==
==Reference==
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[[Category: Protease inhibitor]]
[[Category: Protease inhibitor]]
[[Category: Specificity]]
[[Category: Specificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:47:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:24:09 2008''

Revision as of 07:24, 2 July 2008

Image:1kio.png

Template:STRUCTURE 1kio

SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]

Template:ABSTRACT PUBMED 11856311

About this Structure

1KIO is a Single protein structure. Full experimental information is available from OCA.

Reference

Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria., Gaspari Z, Patthy A, Graf L, Perczel A, Eur J Biochem. 2002 Jan;269(2):527-37. PMID:11856311

Page seeded by OCA on Wed Jul 2 10:24:09 2008

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