7xr2
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==3.1 Angstrom cryoEM icosahedral reconstruction of mud crab reovirus== | ==3.1 Angstrom cryoEM icosahedral reconstruction of mud crab reovirus== | ||
| - | < | + | <SX load='7xr2' size='340' side='right' viewer='molstar' caption='[[7xr2]], [[Resolution|resolution]] 3.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7xr2]] is a 17 chain structure with sequence from [https://en.wikipedia.org/wiki/Scylla_serrata_reovirus_SZ-2007 Scylla serrata reovirus SZ-2007]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XR2 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7xr2]] is a 17 chain structure with sequence from [https://en.wikipedia.org/wiki/Scylla_serrata_reovirus_SZ-2007 Scylla serrata reovirus SZ-2007]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XR2 FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xr2 OCA], [https://pdbe.org/7xr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xr2 RCSB], [https://www.ebi.ac.uk/pdbsum/7xr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xr2 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xr2 OCA], [https://pdbe.org/7xr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xr2 RCSB], [https://www.ebi.ac.uk/pdbsum/7xr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xr2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | [https://www.uniprot.org/uniprot/ | + | [https://www.uniprot.org/uniprot/G9BDA8_9REOV G9BDA8_9REOV] |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Infecting a wide range of hosts, members of Reovirales (formerly Reoviridae) consist of a genome with different numbers of segmented double stranded RNAs (dsRNA) encapsulated by a proteinaceous shell and carry out genome replication and transcription inside the virion. Several cryo-electron microscopy (cryo-EM) structures of reoviruses with 9, 10 or 11 segmented dsRNA genomes have revealed insights into genome arrangement and transcription. However, the structure and genome arrangement of 12-segmented Reovirales members remain poorly understood. Using cryo-EM, we determined the structure of mud crab reovirus (MCRV), a 12-segmented dsRNA virus that is a putative member of Reovirales in the non-turreted Sedoreoviridae family, to near-atomic resolutions with icosahedral symmetry (3.1 A) and without imposing icosahedral symmetry (3.4 A). These structures revealed the organization of the major capsid proteins in two layers: an outer T = 13 layer consisting of VP12 trimers and unique VP11 clamps, and an inner T = 1 layer consisting of VP3 dimers. Additionally, ten RNA dependent RNA polymerases (RdRp) were well resolved just below the VP3 layer but were offset from the 5-fold axes and arranged with D5 symmetry, which has not previously been seen in other members of Reovirales. The N-termini of VP3 were shown to adopt four unique conformations; two of which anchor the RdRps, while the other two conformations are likely involved in genome organization and capsid stability. Taken together, these structures provide a new level of understanding for capsid stabilization and genome organization of segmented dsRNA viruses. | ||
| + | |||
| + | The structure of a 12-segmented dsRNA reovirus: New insights into capsid stabilization and organization.,Zhang Q, Gao Y, Baker ML, Liu S, Jia X, Xu H, He J, Kaelber JT, Weng S, Jiang W PLoS Pathog. 2023 Apr 21;19(4):e1011341. doi: 10.1371/journal.ppat.1011341. , eCollection 2023 Apr. PMID:37083840<ref>PMID:37083840</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7xr2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
| - | </ | + | </SX> |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Scylla serrata reovirus SZ-2007]] | [[Category: Scylla serrata reovirus SZ-2007]] | ||
[[Category: Gao Y]] | [[Category: Gao Y]] | ||
[[Category: Zhang Q]] | [[Category: Zhang Q]] | ||
Current revision
3.1 Angstrom cryoEM icosahedral reconstruction of mud crab reovirus
| |||||||||
