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1klr

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[[Image:1klr.jpg|left|200px]]
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{{STRUCTURE_1klr| PDB=1klr | SCENE= }}
{{STRUCTURE_1klr| PDB=1klr | SCENE= }}
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'''NMR Structure of the ZFY-6T[Y10F] Zinc Finger'''
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===NMR Structure of the ZFY-6T[Y10F] Zinc Finger===
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==Overview==
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The Zn finger provides a model for studies of protein structure and stability. Its core contains a conserved phenylalanine residue adjoining three architectural elements: a beta-hairpin, an alpha-helix and a tetrahedral Zn(2+)-binding site. Here, we demonstrate that the consensus Phe is not required for high-affinity Zn(2+) binding but contributes to the specification of a precise DNA-binding surface. Substitution of Phe by leucine in a ZFY peptide permits Zn(2+)-dependent folding. Although a native-like structure is retained, structural fluctuations lead to attenuation of selected nuclear Overhauser enhancements and accelerated amide proton exchange. Surprisingly, wild-type Zn affinity is maintained by entropy-enthalpy compensation (EEC): a hidden entropy penalty (TDeltaDeltaS 7kcal/mol) is balanced by enhanced enthalpy of association (DeltaDeltaH -7kcal/mol) at 25 degrees C. Because the variant is less well ordered than the Phe-anchored domain, the net change in entropy is opposite to the apparent change in configurational entropy. By analogy to the thermodynamics of organometallic complexation, we propose that EEC arises from differences in solvent reorganization. Exclusion of Leu among biological sequences suggests an evolutionary constraint on the dynamics of a Zn finger.
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(as it appears on PubMed at http://www.pubmed.gov), where 11884136 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11884136}}
==About this Structure==
==About this Structure==
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1KLR is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KLR OCA].
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1KLR is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KLR OCA].
==Reference==
==Reference==
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[[Category: Weiss, M A.]]
[[Category: Weiss, M A.]]
[[Category: Zinc finger]]
[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:53:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:31:39 2008''

Revision as of 07:31, 2 July 2008

Image:1klr.png

Template:STRUCTURE 1klr

NMR Structure of the ZFY-6T[Y10F] Zinc Finger

Template:ABSTRACT PUBMED 11884136

About this Structure

1KLR is a Single protein structure. Full experimental information is available from OCA.

Reference

The hidden thermodynamics of a zinc finger., Lachenmann MJ, Ladbury JE, Phillips NB, Narayana N, Qian X, Weiss MA, J Mol Biol. 2002 Mar 1;316(4):969-89. PMID:11884136

Page seeded by OCA on Wed Jul 2 10:31:39 2008

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