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1kmd

From Proteopedia

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[[Image:1kmd.gif|left|200px]]
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{{STRUCTURE_1kmd| PDB=1kmd | SCENE= }}
{{STRUCTURE_1kmd| PDB=1kmd | SCENE= }}
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'''SOLUTION STRUCTURE OF THE VAM7P PX DOMAIN'''
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===SOLUTION STRUCTURE OF THE VAM7P PX DOMAIN===
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==Overview==
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PX domains have been recently found to act as phosphoinositide binding modules. In the yeast SNARE protein Vam7p, the PX domain binds to PtdIns(3)P and is required for vacuolar targeting. To gain insight into how PX domains function, the solution structure of the ligand-free Vam7p PX domain has been determined by NMR spectroscopy. The Vam7p PX domain has the same overall alpha/beta fold observed in the structures of the ligand-free p47(phox) PX domain and the PtdIns(3)P-bound p40(phox) PX domain, exhibiting several similarities and differences with these two PX domains. Most striking is the similarity between the Vam7p and p40(phox) PX domains in a subset of secondary structure elements despite the low level of sequence identity between them, suggesting that these elements form a conserved core in the PX domain fold. These similarities and the observation that a putative PtdIns(3)P binding site is already formed in the apo Vam7p PX domains suggest that ligand binding does not induce major conformational changes, contrary to what was previously thought. The proposed ligand binding site of the Vam7p PX domain includes basic side chains from the conserved structural core that also participate in PtdIns(3)P binding to the p40(phox) PX domain, and basic side chains from a variable loop that probably inserts into the membrane. These results indicate that PX domains contain a combination of conserved and variable features that allow them to have a common function and at the same time exhibit distinct specificities, mechanisms of regulation, or modes of interaction with effector molecules.
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(as it appears on PubMed at http://www.pubmed.gov), where 11993989 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11993989}}
==About this Structure==
==About this Structure==
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1KMD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KMD OCA].
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1KMD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KMD OCA].
==Reference==
==Reference==
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[[Category: Px domain]]
[[Category: Px domain]]
[[Category: Vam7p]]
[[Category: Vam7p]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:54:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:33:01 2008''

Revision as of 07:33, 2 July 2008

Image:1kmd.png

Template:STRUCTURE 1kmd

SOLUTION STRUCTURE OF THE VAM7P PX DOMAIN

Template:ABSTRACT PUBMED 11993989

About this Structure

1KMD is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

Reference

Solution structure of the Vam7p PX domain., Lu J, Garcia J, Dulubova I, Sudhof TC, Rizo J, Biochemistry. 2002 May 14;41(19):5956-62. PMID:11993989

Page seeded by OCA on Wed Jul 2 10:33:01 2008

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