1jvq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1jvq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jvq, resolution 2.60&Aring;" /> '''Crystal structure a...)
Line 1: Line 1:
-
[[Image:1jvq.gif|left|200px]]<br />
+
[[Image:1jvq.gif|left|200px]]<br /><applet load="1jvq" size="350" color="white" frame="true" align="right" spinBox="true"
-
<applet load="1jvq" size="450" color="white" frame="true" align="right" spinBox="true"
+
caption="1jvq, resolution 2.60&Aring;" />
caption="1jvq, resolution 2.60&Aring;" />
'''Crystal structure at 2.6A of the ternary complex between antithrombin, a P14-P8 reactive loop peptide, and an exogenous tetrapeptide'''<br />
'''Crystal structure at 2.6A of the ternary complex between antithrombin, a P14-P8 reactive loop peptide, and an exogenous tetrapeptide'''<br />
==Overview==
==Overview==
-
Many of the late-onset dementias, including Alzheimer's disease and the, prion encephalopathies, arise from the aberrant aggregation of individual, proteins. The serpin family of serine protease inhibitors provides a, well-defined structural example of such pathological aggregation, as its, mutant variants readily form long-chain polymers, resulting in diseases, ranging from thrombosis to dementia. The intermolecular linkages result, from the insertion of the reactive site loop of one serpin molecule into, the middle strand (s4A) position of the A beta-sheet of another molecule., We define here the structural requirements for small peptides to, competitively bind to and block the s4A position to prevent this, intermolecular linkage and polymerisation. The entry and anchoring of, blocking-peptides is facilitated by the presence of a threonine which, inserts into the site equivalent to P8 of s4A. But the critical, requirement for small blocking-peptides is demonstrated in, crystallographic structures of the complexes formed with selected tri- and, tetrapeptides. These structures indicate that the binding is primarily due, to the insertion of peptide hydrophobic side-chains into the P4 and P6, sites of s4A. The findings allow the rational design of synthetic, blocking-peptides small enough to be suitable for mimetic design. This is, demonstrated here with a tetrapeptide that preferentially blocks the, polymerisation of a pathologically unstable serpin commonly present in, people of European descent.
+
Many of the late-onset dementias, including Alzheimer's disease and the prion encephalopathies, arise from the aberrant aggregation of individual proteins. The serpin family of serine protease inhibitors provides a well-defined structural example of such pathological aggregation, as its mutant variants readily form long-chain polymers, resulting in diseases ranging from thrombosis to dementia. The intermolecular linkages result from the insertion of the reactive site loop of one serpin molecule into the middle strand (s4A) position of the A beta-sheet of another molecule. We define here the structural requirements for small peptides to competitively bind to and block the s4A position to prevent this intermolecular linkage and polymerisation. The entry and anchoring of blocking-peptides is facilitated by the presence of a threonine which inserts into the site equivalent to P8 of s4A. But the critical requirement for small blocking-peptides is demonstrated in crystallographic structures of the complexes formed with selected tri- and tetrapeptides. These structures indicate that the binding is primarily due to the insertion of peptide hydrophobic side-chains into the P4 and P6 sites of s4A. The findings allow the rational design of synthetic blocking-peptides small enough to be suitable for mimetic design. This is demonstrated here with a tetrapeptide that preferentially blocks the polymerisation of a pathologically unstable serpin commonly present in people of European descent.
==About this Structure==
==About this Structure==
-
1JVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NDG, NAG, ACE and NH2 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JVQ OCA].
+
1JVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NDG:'>NDG</scene>, <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVQ OCA].
==Reference==
==Reference==
Line 14: Line 13:
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
-
[[Category: Carrell, R.W.]]
+
[[Category: Carrell, R W.]]
-
[[Category: Huntington, J.A.]]
+
[[Category: Huntington, J A.]]
-
[[Category: Lomas, D.A.]]
+
[[Category: Lomas, D A.]]
-
[[Category: Stein, P.E.]]
+
[[Category: Stein, P E.]]
[[Category: Zhou, A.]]
[[Category: Zhou, A.]]
[[Category: ACE]]
[[Category: ACE]]
Line 26: Line 25:
[[Category: loop-sheet polymer]]
[[Category: loop-sheet polymer]]
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:45:00 2007''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:15 2008''

Revision as of 11:27, 21 February 2008

Image:1jvq.gif

1jvq, resolution 2.60Å

Drag the structure with the mouse to rotate

Crystal structure at 2.6A of the ternary complex between antithrombin, a P14-P8 reactive loop peptide, and an exogenous tetrapeptide

Overview

Many of the late-onset dementias, including Alzheimer's disease and the prion encephalopathies, arise from the aberrant aggregation of individual proteins. The serpin family of serine protease inhibitors provides a well-defined structural example of such pathological aggregation, as its mutant variants readily form long-chain polymers, resulting in diseases ranging from thrombosis to dementia. The intermolecular linkages result from the insertion of the reactive site loop of one serpin molecule into the middle strand (s4A) position of the A beta-sheet of another molecule. We define here the structural requirements for small peptides to competitively bind to and block the s4A position to prevent this intermolecular linkage and polymerisation. The entry and anchoring of blocking-peptides is facilitated by the presence of a threonine which inserts into the site equivalent to P8 of s4A. But the critical requirement for small blocking-peptides is demonstrated in crystallographic structures of the complexes formed with selected tri- and tetrapeptides. These structures indicate that the binding is primarily due to the insertion of peptide hydrophobic side-chains into the P4 and P6 sites of s4A. The findings allow the rational design of synthetic blocking-peptides small enough to be suitable for mimetic design. This is demonstrated here with a tetrapeptide that preferentially blocks the polymerisation of a pathologically unstable serpin commonly present in people of European descent.

About this Structure

1JVQ is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Full crystallographic information is available from OCA.

Reference

How small peptides block and reverse serpin polymerisation., Zhou A, Stein PE, Huntington JA, Sivasothy P, Lomas DA, Carrell RW, J Mol Biol. 2004 Sep 17;342(3):931-41. PMID:15342247

Page seeded by OCA on Thu Feb 21 13:27:15 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jvq"
Personal tools