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1ko3

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{{STRUCTURE_1ko3| PDB=1ko3 | SCENE= }}
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'''VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa with Cys221 reduced'''
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===VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa with Cys221 reduced===
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==Overview==
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The crystal structures of the universally widespread metallo-beta-lactamase (MBL) Verona integron-encoded MBL (VIM)-2 from Pseudomonas aeruginosa have been solved in their native form as well as in an unexpected oxidised form. This carbapenem-hydrolysing enzyme belongs to the so-called B1 subfamily of MBLs and shares the folding of alpha beta/beta alpha sandwich, consisting of a core of beta-sheet surrounded by alpha-helices. Surprisingly, it showed a high tendency to be strongly oxidised at the catalytic cysteine located in the Cys site, Cys221, which, in the oxidised structure, becomes a cysteinesulfonic residue. Its native structure was obtained only in the presence of Tris(2-carboxyethyl)phosphine. This oxidation might be a consequence of a lower affinity for the second Zn located in the Cys site that would also explain the observed susceptibility of VIM-2 to chelating agents. This modification, if present in nature, might play a role in catalytic down-regulation. Comparison between native and oxidised VIM-2 and a predicted model of VIM-1 (which shows one residue different in the Cys site compared with VIM-2) is performed to explain the different activities and antibiotic specificities.
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{{ABSTRACT_PUBMED_18061205}}
==About this Structure==
==About this Structure==
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[[Category: Alpha-beta/beta-alpha fold]]
[[Category: Alpha-beta/beta-alpha fold]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:37:35 2008''

Revision as of 07:37, 2 July 2008

Image:1ko3.png

Template:STRUCTURE 1ko3

VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa with Cys221 reduced

Template:ABSTRACT PUBMED 18061205

About this Structure

1KO3 is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

The three-dimensional structure of VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa in its reduced and oxidised form., Garcia-Saez I, Docquier JD, Rossolini GM, Dideberg O, J Mol Biol. 2008 Jan 18;375(3):604-11. Epub 2007 Nov 13. PMID:18061205

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