1koj

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{{STRUCTURE_1koj| PDB=1koj | SCENE= }}
{{STRUCTURE_1koj| PDB=1koj | SCENE= }}
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'''Crystal structure of rabbit phosphoglucose isomerase complexed with 5-phospho-D-arabinonohydroxamic acid'''
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===Crystal structure of rabbit phosphoglucose isomerase complexed with 5-phospho-D-arabinonohydroxamic acid===
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==Overview==
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Phosphoglucose isomerase (EC ) catalyzes the second step in glycolysis, the reversible isomerization of D-glucose 6-phosphate to D-fructose 6-phosphate. The reaction mechanism involves acid-base catalysis with proton transfer and proceeds through a cis-enediol(ate) intermediate. 5-Phospho-D-arabinonohydroxamic acid (5PAH) is a synthetic small molecule that resembles the reaction intermediate, differing only in that it has a nitrogen atom in place of C1. Hence, 5PAH is the best inhibitor of the isomerization reaction reported to date with a K(i) of 2 x 10(-7) M. Here we report the crystal structure of rabbit phosphoglucose isomerase complexed with 5PAH at 1.9 A resolution. The interaction of 5PAH with amino acid residues in the enzyme active site supports a model of the catalytic mechanism in which Glu-357 transfers a proton between C1 and C2 and Arg-272 helps stabilize the intermediate. It also suggests a mechanism for proton transfer between O1 and O2.
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The line below this paragraph, {{ABSTRACT_PUBMED_11983887}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11983887 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11983887}}
==About this Structure==
==About this Structure==
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[[Category: Salmon, L.]]
[[Category: Salmon, L.]]
[[Category: Protein - inhibitor complex]]
[[Category: Protein - inhibitor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:59:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:39:37 2008''

Revision as of 07:39, 2 July 2008

Image:1koj.png

Template:STRUCTURE 1koj

Crystal structure of rabbit phosphoglucose isomerase complexed with 5-phospho-D-arabinonohydroxamic acid

Template:ABSTRACT PUBMED 11983887

About this Structure

1KOJ is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

The crystal structure of rabbit phosphoglucose isomerase complexed with 5-phospho-D-arabinonohydroxamic acid., Arsenieva D, Hardre R, Salmon L, Jeffery CJ, Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5872-7. PMID:11983887

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