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1kp5

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{{STRUCTURE_1kp5| PDB=1kp5 | SCENE= }}
{{STRUCTURE_1kp5| PDB=1kp5 | SCENE= }}
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'''Cyclic Green Fluorescent Protein'''
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===Cyclic Green Fluorescent Protein===
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==Overview==
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Crystals of cyclic green fluorescent protein (cGFP) engineered by the previously reported split intein technology [Iwai et al. (2001), J. Biol. Chem. 276, 16548-16554] were obtained and the structure was solved using molecular replacement. Although the core of the protein can unambiguously be fitted from the first to the last residue of the genuine sequence, the electron density in the region of the linker peptide is rather poor owing to the high water content of the crystals. Therefore, it is concluded that this part of the protein is highly disordered in the present structure and is very flexible. This is supported by the absence of crystal contacts in the linker-peptide region and the fact that the core of the protein exhibits a very similar conformation to that known from other GFP structures, thereby not implicating any constraints arising from the presence of the artificial linker. Nevertheless, the density is consistent with the loop being intact, as confirmed by mass spectroscopy of dissolved crystals. The present structure contains an antiparallel cGFP dimer where the dimer interface is clearly different from other crystal structures featuring two GFP molecules. This adds further support to the fact that the cylinder surface of GFP is rather versatile and can employ various polar and non-polar patches in protein-protein interactions.
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(as it appears on PubMed at http://www.pubmed.gov), where 12198295 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12198295}}
==About this Structure==
==About this Structure==
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[[Category: Cyclised termini]]
[[Category: Cyclised termini]]
[[Category: Green fluorescent protein]]
[[Category: Green fluorescent protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:00:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:41:36 2008''

Revision as of 07:41, 2 July 2008

Image:1kp5.png

Template:STRUCTURE 1kp5

Cyclic Green Fluorescent Protein

Template:ABSTRACT PUBMED 12198295

About this Structure

1KP5 is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.

Reference

Structure of cyclized green fluorescent protein., Hofmann A, Iwai H, Hess S, Pluckthun A, Wlodawer A, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1400-6. Epub 2002, Aug 23. PMID:12198295

Page seeded by OCA on Wed Jul 2 10:41:36 2008

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