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| | {{STRUCTURE_1kpd| PDB=1kpd | SCENE= }} | | {{STRUCTURE_1kpd| PDB=1kpd | SCENE= }} |
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| - | '''A MUTANT RNA PSEUDOKNOT THAT PROMOTES RIBOSOMAL FRAMESHIFTING IN MOUSE MAMMARY TUMOR VIRUS, NMR, MINIMIZED AVERAGE STRUCTURE'''
| + | ===A MUTANT RNA PSEUDOKNOT THAT PROMOTES RIBOSOMAL FRAMESHIFTING IN MOUSE MAMMARY TUMOR VIRUS, NMR, MINIMIZED AVERAGE STRUCTURE=== |
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| - | ==Overview==
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| - | A single A-->G mutation that changes a potential A.U base pair to a G.U pair at the junction of the stems and loops of a non-frameshifting pseudoknot dramatically increases its frameshifting efficiency in mouse mammary tumor virus. The structure of the non-frameshifting pseudoknot APK has been found to be very different from that of pseudoknots that cause efficient frameshifting [Kang,H., Hines,J.V. and Tinoco,I. (1995) J. Mol. Biol. , 259, 135-147]. The 3-dimensional structure of the mutant pseudoknot was determined by restrained molecular dynamics based on NMR-derived interproton distance and torsion angle constraints. One striking feature of the mutant pseudoknot compared with the parent pseudoknot is that a G.U base pair forms at the top of stem 2, thus leaving only 1 nt at the junction of the two stems. The conformation is very different from that of the previously determined non-frameshifting parent pseudoknot, which lacks the A.U base pair at the top of the stem and has 2 nt between the stems. However, the conformation is quite similar to that of efficient frameshifting pseudoknots whose structures were previously determined by NMR. A single adenylate residue intervenes between the two stems and interrupts their coaxial stacking. This unpaired nucleotide produces a bent structure. The structural similarity among the efficient frameshifting pseudoknots indicates that a specific conformation is required for ribosomal frameshifting, further implying a specific interaction of the pseudoknot with the ribosome.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9115361}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9115361 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9115361}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KPD OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KPD OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Ribonucleic acid]] | | [[Category: Ribonucleic acid]] |
| | [[Category: Rna pseudoknot]] | | [[Category: Rna pseudoknot]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:00:45 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:42:30 2008'' |
Revision as of 07:42, 2 July 2008
Template:STRUCTURE 1kpd
A MUTANT RNA PSEUDOKNOT THAT PROMOTES RIBOSOMAL FRAMESHIFTING IN MOUSE MAMMARY TUMOR VIRUS, NMR, MINIMIZED AVERAGE STRUCTURE
Template:ABSTRACT PUBMED 9115361
About this Structure
Full experimental information is available from OCA.
Reference
A mutant RNA pseudoknot that promotes ribosomal frameshifting in mouse mammary tumor virus., Kang H, Tinoco I Jr, Nucleic Acids Res. 1997 May 15;25(10):1943-9. PMID:9115361
Page seeded by OCA on Wed Jul 2 10:42:30 2008
