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4yk6

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Current revision

Crystal structure of APC-ARM in complexed with Amer1-A4

Structural highlights

4yk6 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

AMER1_HUMAN Osteopathia striata - cranial sclerosis. The disease is caused by mutations affecting the gene represented in this entry.

Function

AMER1_HUMAN Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta-catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development.^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

The tumor suppressor APC employs its conserved armadillo repeat (ARM) domain to recognize many of its binding partners, including Amer1/WTX, which is mutated in Wilms' tumor and bone overgrowth syndrome. The APC-Amer1 complex has important roles in regulating Wnt signaling and cell adhesion. Three sites A1, A2, and A3 of Amer1 have been reported to mediate its interaction with APC-ARM. In this study, crystal structures of APC-ARM in complexes with Amer1-A1, -A2, and -A4, which is newly identified in this work, were determined. Combined with our GST pull-down, yeast two-hybrid, and isothermal titration calorimetry (ITC) assay results using mutants of APC and Amer1 interface residues, our structures demonstrate that Amer1-A1, -A2, and -A4, as well as other APC-binding proteins such as Asef and Sam68, all employ a common recognition pattern to associate with APC-ARM. In contrast, Amer1-A3 binds to the C-terminal side of APC-ARM through a bipartite interaction mode. Composite mutations on either APC or Amer1 disrupting all four interfaces abrogated their association in cultured cells and impaired the membrane recruitment of APC by Amer1. Our study thus comprehensively elucidated the recognition mechanism between APC and Amer1, and revealed a consensus recognition sequence employed by various APC-ARM binding partners.

Structures of the APC-ARM domain in complexes with discrete Amer1/WTX fragments reveal that it uses a consensus mode to recognize its binding partners.,Zhang Z, Akyildiz S, Xiao Y, Gai Z, An Y, Behrens J, Wu G Cell Discov. 2015 Jul 14;1:15016. doi: 10.1038/celldisc.2015.16. eCollection, 2015. PMID:27462415^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Major MB, Camp ND, Berndt JD, Yi X, Goldenberg SJ, Hubbert C, Biechele TL, Gingras AC, Zheng N, Maccoss MJ, Angers S, Moon RT. Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling. Science. 2007 May 18;316(5827):1043-6. PMID:17510365 doi:http://dx.doi.org/10.1126/science/1141515
↑ Grohmann A, Tanneberger K, Alzner A, Schneikert J, Behrens J. AMER1 regulates the distribution of the tumor suppressor APC between microtubules and the plasma membrane. J Cell Sci. 2007 Nov 1;120(Pt 21):3738-47. Epub 2007 Oct 9. PMID:17925383 doi:http://dx.doi.org/10.1242/jcs.011320
↑ Rivera MN, Kim WJ, Wells J, Stone A, Burger A, Coffman EJ, Zhang J, Haber DA. The tumor suppressor WTX shuttles to the nucleus and modulates WT1 activity. Proc Natl Acad Sci U S A. 2009 May 19;106(20):8338-43. doi:, 10.1073/pnas.0811349106. Epub 2009 May 4. PMID:19416806 doi:10.1073/pnas.0811349106
↑ Tanneberger K, Pfister AS, Brauburger K, Schneikert J, Hadjihannas MV, Kriz V, Schulte G, Bryja V, Behrens J. Amer1/WTX couples Wnt-induced formation of PtdIns(4,5)P2 to LRP6 phosphorylation. EMBO J. 2011 Apr 20;30(8):1433-43. doi: 10.1038/emboj.2011.28. Epub 2011 Feb 8. PMID:21304492 doi:http://dx.doi.org/10.1038/emboj.2011.28
↑ Tanneberger K, Pfister AS, Kriz V, Bryja V, Schambony A, Behrens J. Structural and functional characterization of the Wnt inhibitor APC membrane recruitment 1 (Amer1). J Biol Chem. 2011 Jun 3;286(22):19204-14. doi: 10.1074/jbc.M111.224881. Epub 2011, Apr 15. PMID:21498506 doi:http://dx.doi.org/10.1074/jbc.M111.224881
↑ Zhang Z, Akyildiz S, Xiao Y, Gai Z, An Y, Behrens J, Wu G. Structures of the APC-ARM domain in complexes with discrete Amer1/WTX fragments reveal that it uses a consensus mode to recognize its binding partners. Cell Discov. 2015 Jul 14;1:15016. doi: 10.1038/celldisc.2015.16. eCollection, 2015. PMID:27462415 doi:http://dx.doi.org/10.1038/celldisc.2015.16

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4yk6"

Categories: Homo sapiens | Large Structures | Wu G | Xiao Y | Zhang Z

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4yk6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YK6 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yk6 OCA], [https://pdbe.org/4yk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yk6 RCSB], [https://www.ebi.ac.uk/pdbsum/4yk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yk6 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yk6 OCA], [https://pdbe.org/4yk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yk6 RCSB], [https://www.ebi.ac.uk/pdbsum/4yk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yk6 ProSAT]</span></td></tr>
 </table>
 == Disease ==

4yk6

From Proteopedia

Current revision

Crystal structure of APC-ARM in complexed with Amer1-A4

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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