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| - | [[Image:1ks8.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1ks8| PDB=1ks8 | SCENE= }} | | {{STRUCTURE_1ks8| PDB=1ks8 | SCENE= }} |
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| - | '''The structure of Endoglucanase from termite, Nasutitermes takasagoensis, at pH 2.5.'''
| + | ===The structure of Endoglucanase from termite, Nasutitermes takasagoensis, at pH 2.5.=== |
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| - | ==Overview==
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| - | Contrary to conventional wisdom, it has been shown recently that termites do not necessarily depend on symbiotic bacteria to process cellulose. They secrete their own cellulases, mainly endo-beta-1,4-glucanase and beta-1,4-glucosidase. Here, the first structure of an endogenous endoglucanase from the higher termite Nasutitermes takasagoensis (NtEgl) is reported at 1.40 A resolution. NtEgl has the general folding of an (alpha/alpha)(6) barrel, which is a common folding pattern for glycosyl hydrolase family 9. Three-dimensional structural analysis shows that the conserved Glu412 is the catalytic acid/base residue and the conserved Asp54 or Asp57 is the base. The enzyme has a Ca(2+)-binding site near its substrate-binding cleft. Comparison between the structure of the Ca(2+)-free enzyme produced by reducing the pH of the soaked crystal from 5.6 (the pH of optimum enzyme activity) to 2.5 with that of the Ca(2+)-bound enzyme did not show significant differences in the locations of the C(alpha) atoms. The main differences are in the conformation of the residue side chains ligating the Ca(2+) ion. The overall structure of NtEgl at pH 6.5 is similar to that at pH 5.6. The major change observed was in the conformation of the side chain of the catalytic acid/base Glu412, which rotates from a hydrophobic cavity to a relatively hydrophilic environment. This side-chain displacement may decrease the enzyme activity at higher pH.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11914490}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11914490 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11914490}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Nasutitermes takasagoensis]] | | [[Category: Nasutitermes takasagoensis]] |
| | [[Category: Termite]] | | [[Category: Termite]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:06:20 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:53:05 2008'' |
Revision as of 07:53, 2 July 2008
Template:STRUCTURE 1ks8
The structure of Endoglucanase from termite, Nasutitermes takasagoensis, at pH 2.5.
Template:ABSTRACT PUBMED 11914490
About this Structure
1KS8 is a Single protein structure of sequence from Nasutitermes takasagoensis. Full crystallographic information is available from OCA.
Reference
Structure of an endoglucanase from termite, Nasutitermes takasagoensis., Khademi S, Guarino LA, Watanabe H, Tokuda G, Meyer EF, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):653-9. Epub 2002, Mar 22. PMID:11914490
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