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4zk7
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4zk7]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZK7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4zk7]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZK7 FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zk7 OCA], [https://pdbe.org/4zk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zk7 RCSB], [https://www.ebi.ac.uk/pdbsum/4zk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zk7 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zk7 OCA], [https://pdbe.org/4zk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zk7 RCSB], [https://www.ebi.ac.uk/pdbsum/4zk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zk7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/Q5SJY4_THET8 Q5SJY4_THET8] | [https://www.uniprot.org/uniprot/Q5SJY4_THET8 Q5SJY4_THET8] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | We recently reported the development of a computational method for the design of co-assembling, multi-component protein nanomaterials. While four such materials were validated at high-resolution by X-ray crystallography, low yield of soluble protein prevented X-ray structure determination of a fifth designed material, T33-09. Here we report the design and crystal structure of T33-31, a variant of T33-09 with improved soluble yield resulting from redesign efforts focused on mutating solvent-exposed side chains to charged amino acids. The structure is found to match the computational design model with atomic-level accuracy, providing further validation of the design approach and demonstrating a simple and potentially general means of improving the yield of designed protein nanomaterials. This article is protected by copyright. All rights reserved. | ||
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| - | Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression.,Bale JB, Park RU, Liu Y, Gonen S, Gonen T, Cascio D, King NP, Yeates TO, Baker D Protein Sci. 2015 Jul 15. doi: 10.1002/pro.2748. PMID:26174163<ref>PMID:26174163</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 4zk7" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[CutA1 3D structures|CutA1 3D structures]] | *[[CutA1 3D structures|CutA1 3D structures]] | ||
*[[3D structures of chorismate mutase|3D structures of chorismate mutase]] | *[[3D structures of chorismate mutase|3D structures of chorismate mutase]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal structure of rescued two-component self-assembling tetrahedral cage T33-31
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Categories: Large Structures | Thermus thermophilus HB8 | Baker D | Bale J | Cascio D | Collazo MJ | King N | Liu Y | Park R | Sawaya MR | Yeates T
