4zua

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zua OCA], [https://pdbe.org/4zua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zua RCSB], [https://www.ebi.ac.uk/pdbsum/4zua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zua ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Pseudomonas aeruginosa employs a type three secretion system to facilitate infections in mammalian hosts. The operons encoding genes of structural components of the secretion machinery and associated virulence factors are all under the control of the AraC-type transcriptional activator protein, ExsA. ExsA belongs to a unique subfamily of AraC-proteins that is regulated through protein-protein contacts rather than small molecule ligands. Prior to infection, ExsA is inhibited through a direct interaction with the anti-activator ExsD. To activate ExsA upon host cell contact this interaction is disrupted by the anti-antiactivator protein ExsC. Here we report the crystal structure of the regulatory domain of ExsA, which is known to mediate ExsA dimerization as well as ExsD binding. The crystal structure suggests two models for the ExsA dimer. Both models confirmed the previously shown involvement of helix alpha-3 in ExsA dimerization but one also suggest a role for helix alpha-2. These structural data are supported by the observation that a mutation in alpha-2 greatly diminished the ability of ExsA to activate transcription in vitro. Additional in vitro transcription studies revealed that a conserved pocket, used by AraC and the related ToxT protein for the binding of small molecule regulators, although present in ExsA is not involved in binding of ExsD.

Structural Analysis of the Regulatory Domain of ExsA, a Key Transcriptional Regulator of the Type Three Secretion System in Pseudomonas aeruginosa.,Shrestha M, Xiao Y, Robinson H, Schubot FD PLoS One. 2015 Aug 28;10(8):e0136533. doi: 10.1371/journal.pone.0136533., eCollection 2015. PMID:26317977<ref>PMID:26317977</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

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