1ku7

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{{STRUCTURE_1ku7| PDB=1ku7 | SCENE= }}
{{STRUCTURE_1ku7| PDB=1ku7 | SCENE= }}
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'''Crystal Structure of Thermus aquatics RNA Polymerase SigmaA Subunit Region 4 Bound to-35 Element DNA'''
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===Crystal Structure of Thermus aquatics RNA Polymerase SigmaA Subunit Region 4 Bound to-35 Element DNA===
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==Overview==
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The sigma subunit is the key regulator of bacterial transcription. Proteolysis of Thermus aquaticus sigma(A), which occurred in situ during crystallization, reveals three domains, sigma(2), sigma(3), and sigma(4), connected by flexible linkers. Crystal structures of each domain were determined, as well as of sigma(4) complexed with -35 element DNA. Exposed surfaces of each domain are important for RNA polymerase binding. Universally conserved residues important for -10 element recognition and melting lie on one face of sigma(2), while residues important for extended -10 recognition lie on sigma(3). Genetic studies correctly predicted that a helix-turn-helix motif in sigma(4) recognizes the -35 element but not the details of the protein-DNA interactions. Positive control mutants in sigma(4) cluster in two regions, positioned to interact with activators bound just upstream or downstream of the -35 element.
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(as it appears on PubMed at http://www.pubmed.gov), where 11931761 is the PubMed ID number.
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==About this Structure==
==About this Structure==
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[[Category: Double-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:10:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:00:47 2008''

Revision as of 08:00, 2 July 2008

Image:1ku7.png

Template:STRUCTURE 1ku7

Crystal Structure of Thermus aquatics RNA Polymerase SigmaA Subunit Region 4 Bound to-35 Element DNA

Template:ABSTRACT PUBMED 11931761

About this Structure

1KU7 is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.

Reference

Structure of the bacterial RNA polymerase promoter specificity sigma subunit., Campbell EA, Muzzin O, Chlenov M, Sun JL, Olson CA, Weinman O, Trester-Zedlitz ML, Darst SA, Mol Cell. 2002 Mar;9(3):527-39. PMID:11931761

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