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| | {{STRUCTURE_1kvz| PDB=1kvz | SCENE= }} | | {{STRUCTURE_1kvz| PDB=1kvz | SCENE= }} |
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| - | '''Solution Structure of Cytotoxic RC-RNase4'''
| + | ===Solution Structure of Cytotoxic RC-RNase4=== |
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| - | ==Overview==
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| - | Cytotoxic ribonucleases with antitumor activity are mainly found in the oocytes and early embryos of frogs. Native RC-RNase 4 (RNase 4), consisting of 106 residues linked with four disulfide bridges, is a cytotoxic ribonuclease isolated from oocytes of bullfrog Rana catesbeiana. RNase 4 belongs to the bovine pancreatic ribonuclease (RNase A) superfamily. Recombinant RC-RNase 4 (rRNase 4), which contains an additional Met residue and glutamine instead of pyroglutamate at the N terminus, was found to possess less catalytic and cytotoxic activities than RNase 4. Equilibrium thermal and guanidine-HCl denaturation CD measurements revealed that RNase 4 is more thermally and chemically stable than rRNase 4. However, CD and NMR data showed that there is no gross conformational change between native and recombinant RNase 4. The NMR solution structure of rRNase 4 was determined to comprise three alpha-helices and two sets of antiparallel beta-sheets. Superimposition of each structure with the mean structure yielded an average root mean square deviation (RMSD) of 0.72(+/-0.14)A for the backbone atoms, and 1.42(+/-0.19)A for the heavy atoms in residues 3-105. A comparison of the 3D structure of rRNase 4 with the structurally and functionally related cytotoxic ribonuclease, onconase (ONC), showed that the two H-bonds in the N-terminal pyroglutamate of ONC were not present at the corresponding glutamine residue of rRNase 4. We suggest that the loss of these two H-bonds is one of the key factors responsible for the reductions of the conformational stability, catalytic and cytotoxic activities in rRNase 4. Furthermore, the differences of side-chain conformations of subsite residues among RNase A, ONC and rRNase 4 are related to their distinct catalytic activities and base preferences.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12589762}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12589762 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12589762}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1KVZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KVZ OCA]. | + | 1KVZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KVZ OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Nmr]] | | [[Category: Nmr]] |
| | [[Category: Structure from molmol]] | | [[Category: Structure from molmol]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:14:06 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:07:38 2008'' |
Revision as of 08:07, 2 July 2008
Template:STRUCTURE 1kvz
Solution Structure of Cytotoxic RC-RNase4
Template:ABSTRACT PUBMED 12589762
About this Structure
1KVZ is a Single protein structure of sequence from Rana catesbeiana. Full experimental information is available from OCA.
Reference
Solution structure of the cytotoxic RNase 4 from oocytes of bullfrog Rana catesbeiana., Hsu CH, Liao YD, Pan YR, Chen LW, Wu SH, Leu YJ, Chen C, J Mol Biol. 2003 Feb 28;326(4):1189-201. PMID:12589762
Page seeded by OCA on Wed Jul 2 11:07:38 2008
