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5aoy

From Proteopedia

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Structure of mouse Endonuclease V

Structural highlights

5aoy is a 1 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.75Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENDOV_MOUSE Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures (By similarity).

Publication Abstract from PubMed

Endonuclease V (EndoV) is an enzyme with specificity for deaminated adenosine (inosine) in nucleic acids. EndoV from Escherichia coli (EcEndoV) acts both on inosines in DNA and RNA, whereas the human homolog cleaves only at inosines in RNA. Inosines in DNA are mutagenic and the role of EndoV in DNA repair is well established. In contrast, the biological function of EndoV in RNA processing is largely unexplored. Here we have characterized a second mammalian EndoV homolog, mouse EndoV (mEndoV), and show that mEndoV shares the same RNA selectivity as human EndoV (hEndoV). Mouse EndoV cleaves the same inosine-containing substrates as hEndoV, but with reduced efficiencies. The crystal structure of mEndoV reveals a conformation different from the hEndoV and prokaryotic EndoV structures, particularly for the conserved tyrosine in the wedge motif, suggesting that this strand separating element has some flexibility. Molecular dynamics simulations of mouse and human EndoV reveal alternative conformations for the invariant tyrosine. The configuration of the active site, on the other hand, is very similar between the prokaryotic and mammalian versions of EndoV.

Crystal structure and MD simulation of mouse EndoV reveal wedge motif plasticity in this inosine-specific endonuclease.,Nawaz MS, Vik ES, Ronander ME, Solvoll AM, Blicher P, Bjoras M, Alseth I, Dalhus B Sci Rep. 2016 Apr 25;6:24979. doi: 10.1038/srep24979. PMID:27108838^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nawaz MS, Vik ES, Ronander ME, Solvoll AM, Blicher P, Bjoras M, Alseth I, Dalhus B. Crystal structure and MD simulation of mouse EndoV reveal wedge motif plasticity in this inosine-specific endonuclease. Sci Rep. 2016 Apr 25;6:24979. doi: 10.1038/srep24979. PMID:27108838 doi:http://dx.doi.org/10.1038/srep24979

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5aoy"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5aoy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AOY FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aoy OCA], [https://pdbe.org/5aoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aoy RCSB], [https://www.ebi.ac.uk/pdbsum/5aoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aoy ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aoy OCA], [https://pdbe.org/5aoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aoy RCSB], [https://www.ebi.ac.uk/pdbsum/5aoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aoy ProSAT]</span></td></tr>
 </table>
 == Function ==

5aoy

From Proteopedia

Current revision

Structure of mouse Endonuclease V

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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