1l2k

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{{STRUCTURE_1l2k| PDB=1l2k | SCENE= }}
{{STRUCTURE_1l2k| PDB=1l2k | SCENE= }}
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'''Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.'''
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===Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.===
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==Overview==
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From the first days of protein neutron structure determination sperm whale myoglobin was an object under investigation [Nature 224 (1969) 143, J. Mol. Biol. 220 (1991) 381]. Nevertheless myoglobin is still of interest [Proc. Natl. Acad. Sci. USA 97 (2000) 3872]. The feasibility of the monochromatic neutron diffractometer BIX-3 at the JRR-3M reactor at the JAERI [J. Phys. Chem. Solids 60 (1999) 1623], to collect high-resolution diffraction data in a relatively short time stimulated us to repeat the structural determination of myoglobin. The structure of metmyoglobin has been determined up to a resolution of 1.5 A. The hydrogen atoms were replaced in part, by deuterium soaking the crystals for more than 10 years in D(2)O. A refinement of all atoms has been performed including the refinement of individual mean square displacements and occupancies of the exchangeable protons in backbone hydrogen bonds. A method is described to show clear negative scattering densities of the H atoms. Water molecules within the protein and on the molecule surface are shown. The exchangeability of H atoms is correlated with structural distribution and flexibility.
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(as it appears on PubMed at http://www.pubmed.gov), where 12062378 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12062378}}
==About this Structure==
==About this Structure==
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[[Category: Hydrogen atom]]
[[Category: Hydrogen atom]]
[[Category: Neutron structure]]
[[Category: Neutron structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:28:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:33:30 2008''

Revision as of 08:33, 2 July 2008

Image:1l2k.png

Template:STRUCTURE 1l2k

Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.

Template:ABSTRACT PUBMED 12062378

About this Structure

1L2K is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.

Reference

Hydrogen and deuterium in myoglobin as seen by a neutron structure determination at 1.5 A resolution., Ostermann A, Tanaka I, Engler N, Niimura N, Parak FG, Biophys Chem. 2002 Mar 28;95(3):183-93. PMID:12062378

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