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1l3m

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{{STRUCTURE_1l3m| PDB=1l3m | SCENE= }}
{{STRUCTURE_1l3m| PDB=1l3m | SCENE= }}
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'''The Solution Structure of [d(CGC)r(amamam)d(TTTGCG)]2'''
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===The Solution Structure of [d(CGC)r(amamam)d(TTTGCG)]2===
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==Overview==
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The solution structure and hydration of a DNA.RNA hybrid chimeric duplex [d(CGC)r(amamam)d(TTTGCG)]2 in which the RNA adenines were substituted by 2'-O-methylated riboadenines was determined using two-dimensional NMR, simulated annealing, and restrained molecular dynamics. Only DNA residue 7T in the 2'-OMe-RNA.DNA junction adopted an O4'-endo sugar conformation, while the other DNA residues including 3C in the DNA.2'-OMe-RNA junction, adopted C1'-exo or C2'-endo conformations. The observed NOE intensity of 2'-O-methyl group to H1' proton of 4am at the DNA.2'-OMe-RNA junction is much weaker than those of 5am and 6am. The 2'-O-methyl group of 4am was found to orient towards the minor groove in the trans domain while the 2'-O-methyl groups of 5am and 6am were found to be in the gauche (+) domain. In contrast to the long-lived water molecules found close to the RNA adenine H2 and H1' protons and the methyl group of 7T in the RNA-DNA junction of [d(CGC)r(aaa)d(TTTGCG)]2, there were no long-lived water molecules found in [d(CGC)r(amamam)d(TTTGCG)]2. This is probably due to the hydrophobic enviroment created by the 2'-O-methylated riboadenines in the minor groove or due to the wider minor groove width in the middle of the structure. In addition, the 2'-O-methylation of riboadenines in pure chimeric duplex increses its melting temperature from 48.5 degrees C to 51.9 degrees C. The characteristic structural features and hydration patterns of this chimeric duplex provide a molecular basis for further therapeutic applications of DNA.RNA hybrid and chimeric duplexes with 2'-modified RNA residues.
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(as it appears on PubMed at http://www.pubmed.gov), where 11775738 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11775738}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3M OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3M OCA].
==Reference==
==Reference==
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[[Category: Wang, L Y.]]
[[Category: Wang, L Y.]]
[[Category: Dna/rna hybrid,chimeric duplex,2'o-methyl]]
[[Category: Dna/rna hybrid,chimeric duplex,2'o-methyl]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:30:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:37:43 2008''

Revision as of 08:37, 2 July 2008

Image:1l3m.png

Template:STRUCTURE 1l3m

The Solution Structure of [d(CGC)r(amamam)d(TTTGCG)]2

Template:ABSTRACT PUBMED 11775738

About this Structure

Full experimental information is available from OCA.

Reference

The solution structure of [d(CGC)r(amamam)d(TTTGCG)]2., Tsao YP, Wang LY, Hsu ST, Jain ML, Chou SH, Huang C, Cheng JW, J Biomol NMR. 2001 Nov;21(3):209-20. PMID:11775738

Page seeded by OCA on Wed Jul 2 11:37:43 2008

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