1kmq
From Proteopedia
(New page: 200px<br /> <applet load="1kmq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kmq, resolution 1.55Å" /> '''Crystal Structure o...) |
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| - | [[Image:1kmq.gif|left|200px]]<br /> | + | [[Image:1kmq.gif|left|200px]]<br /><applet load="1kmq" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1kmq" size=" | + | |
caption="1kmq, resolution 1.55Å" /> | caption="1kmq, resolution 1.55Å" /> | ||
'''Crystal Structure of a Constitutively Activated RhoA Mutant (Q63L)'''<br /> | '''Crystal Structure of a Constitutively Activated RhoA Mutant (Q63L)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Mutants of the small G protein RhoA that are deficient in GTPase activity | + | Mutants of the small G protein RhoA that are deficient in GTPase activity and thereby exhibit constitutive molecular signaling activity are commonly used to discover its cellular functions. In particular, two such mutants, Gly14-->Val (G14V) and Gln63-->Leu (Q63L), are often used interchangeably for such studies. However, while their in vitro rates of GTP hydrolysis are very similar, differences are observed in their other functional properties. The structure of G14V-RhoA is known; in order to assess whether structural variations are responsible for functional differences, the crystal structure of a Q63L-RhoA bound to the GTP-analog 5'-guanylylimidodiphosphate (GMPPNP) was determined at 1.5 A resolution. Overall, the structure is very similar to that of G14V-RhoA, but the significantly higher resolution data permit an improved basis for structural analysis and comparison. The data support the notion that differences observed between the mutants in vivo are likely to arise from altered affinities for RhoGDI and not from direct structural differences. |
==About this Structure== | ==About this Structure== | ||
| - | 1KMQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, GNP and DIO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1KMQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=GNP:'>GNP</scene> and <scene name='pdbligand=DIO:'>DIO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KMQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Derewenda, Z | + | [[Category: Derewenda, Z S.]] |
| - | [[Category: Lin, S | + | [[Category: Lin, S K.]] |
[[Category: Longenecker, K.]] | [[Category: Longenecker, K.]] | ||
| - | [[Category: Nakamoto, R | + | [[Category: Nakamoto, R K.]] |
[[Category: Read, P.]] | [[Category: Read, P.]] | ||
| - | [[Category: Somlyo, A | + | [[Category: Somlyo, A P.]] |
[[Category: DIO]] | [[Category: DIO]] | ||
[[Category: GNP]] | [[Category: GNP]] | ||
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[[Category: gtpase]] | [[Category: gtpase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:35:49 2008'' |
Revision as of 11:35, 21 February 2008
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Crystal Structure of a Constitutively Activated RhoA Mutant (Q63L)
Overview
Mutants of the small G protein RhoA that are deficient in GTPase activity and thereby exhibit constitutive molecular signaling activity are commonly used to discover its cellular functions. In particular, two such mutants, Gly14-->Val (G14V) and Gln63-->Leu (Q63L), are often used interchangeably for such studies. However, while their in vitro rates of GTP hydrolysis are very similar, differences are observed in their other functional properties. The structure of G14V-RhoA is known; in order to assess whether structural variations are responsible for functional differences, the crystal structure of a Q63L-RhoA bound to the GTP-analog 5'-guanylylimidodiphosphate (GMPPNP) was determined at 1.5 A resolution. Overall, the structure is very similar to that of G14V-RhoA, but the significantly higher resolution data permit an improved basis for structural analysis and comparison. The data support the notion that differences observed between the mutants in vivo are likely to arise from altered affinities for RhoGDI and not from direct structural differences.
About this Structure
1KMQ is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 A resolution., Longenecker K, Read P, Lin SK, Somlyo AP, Nakamoto RK, Derewenda ZS, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):876-80. Epub 2003, Apr 25. PMID:12777804
Page seeded by OCA on Thu Feb 21 13:35:49 2008
Categories: Homo sapiens | Single protein | Derewenda, Z S. | Lin, S K. | Longenecker, K. | Nakamoto, R K. | Read, P. | Somlyo, A P. | DIO | GNP | MG | Constitutive mutant | Gtp-analog | Gtpase
