2n8f
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2n8f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hadronyche_infensa Hadronyche infensa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8F FirstGlance]. <br> | <table><tr><td colspan='2'>[[2n8f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hadronyche_infensa Hadronyche infensa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8F FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8f OCA], [https://pdbe.org/2n8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8f RCSB], [https://www.ebi.ac.uk/pdbsum/2n8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8f ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8f OCA], [https://pdbe.org/2n8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8f RCSB], [https://www.ebi.ac.uk/pdbsum/2n8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8f ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == Function == | ||
| - | [https://www.uniprot.org/uniprot/TP1A_HADIN TP1A_HADIN] This toxin potently and selectively inhibits ASIC1a (IC(50)=0.4 nM on rASIC1a and IC(50)=0.52 nM on hASIC1a), an isoform of the gene ASIC1 (PubMed:28320941). It incompletely inhibits ASIC1a activation in a pH-independent and slowly reversible manner (Tau(off)=14.2 min for rASIC1a and 31.8 min for hASIC1a) (PubMed:28320941). This toxin acts by binding to and stabilizing the closed state of the channel, thereby impeding the transition into a conducting state (PubMed:28320941). This toxin may bind to the acidic pocket of ASIC1a, since mutation of a key residue of this pocket (Arg-350) abolishes the ability of the toxin to inhibit ASIC1a (PubMed:28320941). In addition, it shows antiparasitic activities, since it moderately inhibits the larval development of the major pathogenic nematode of ruminants (H.contortus, IC(50)=22.9 uM) (PubMed:31213240). In vivo, this toxin protects the brain from neuronal injury when administered up to 8 hours after stroke onset (PubMed:28320941).<ref>PMID:28320941</ref> <ref>PMID:31213240</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
Current revision
Chemical shift assignments and structure calculation of spider toxin pi-hexatoxin-Hi1a
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