1kpq
From Proteopedia
(New page: 200px<br /> <applet load="1kpq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kpq" /> '''Structure of the Tsg101 UEV domain'''<br />...) |
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'''Structure of the Tsg101 UEV domain'''<br /> | '''Structure of the Tsg101 UEV domain'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KPQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1KPQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KPQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: e2 fold]] | [[Category: e2 fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:14:51 2008'' |
Revision as of 14:14, 15 February 2008
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Structure of the Tsg101 UEV domain
Contents |
Overview
Human Tsg101 plays key roles in HIV budding and in cellular vacuolar, protein sorting (VPS). In performing these functions, Tsg101 binds both, ubiquitin (Ub) and the PTAP tetrapeptide 'late domain' motif located, within the viral Gag protein. These interactions are mediated by the, N-terminal domain of Tsg101, which belongs to the catalytically inactive, ubiquitin E2 variant (UEV) family. We now report the structure of Tsg101, UEV and chemical shift mapping of the Ub and PTAP binding sites. Tsg101, UEV resembles canonical E2 ubiquitin conjugating enzymes, but has an, additional N-terminal helix, an extended beta-hairpin that links strands 1, and 2, and lacks the two C-terminal helices normally found in E2 enzymes., PTAP-containing peptides bind in a hydrophobic cleft exposed by the, absence of the C-terminal helices, whereas ubiquitin binds in a novel site, surrounding the beta-hairpin. These studies provide a structural framework, for understanding how Tsg101 mediates the protein-protein interactions, required for HIV budding and VPS.
Disease
Known disease associated with this structure: Breast cancer OMIM:[601387]
About this Structure
1KPQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and functional interactions of the Tsg101 UEV domain., Pornillos O, Alam SL, Rich RL, Myszka DG, Davis DR, Sundquist WI, EMBO J. 2002 May 15;21(10):2397-406. PMID:12006492
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