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8jch

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Current revision (11:42, 1 February 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 8jch is ON HOLD until 2025-05-11
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==Cryo-EM structure of yeast Rat1-bound Pol II pre-termination transcription complex 1 (Pol II Rat1-PTTC1)==
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<StructureSection load='8jch' size='340' side='right'caption='[[8jch]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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Authors: Zeng, Y., Zhang, Y.
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8jch]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JCH FirstGlance]. <br>
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Description: RNA Polymerase II pre-termination complex bound with Rat1-Rai1 and Spt5
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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[[Category: Unreleased Structures]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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[[Category: Zhang, Y]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jch OCA], [https://pdbe.org/8jch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jch RCSB], [https://www.ebi.ac.uk/pdbsum/8jch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jch ProSAT]</span></td></tr>
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[[Category: Zeng, Y]]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DXO_YEAST DXO_YEAST] Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA (By similarity). The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (By similarity). Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs (PubMed:12897126, PubMed:20802481, PubMed:26101253). Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1) (PubMed:12897126, PubMed:20802481). Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates (PubMed:20802481). Stimulates exoribonuclease activity of RAT1, allowing it to degrade RNAs with stable secondary structure more effectively (PubMed:20802481). Required for the processing of nuclear mRNA and rRNA precursors (PubMed:10805743, PubMed:12612077, PubMed:16131592). May promote termination of transcription by RNA polymerase II (PubMed:15565157).[UniProtKB:O13836][UniProtKB:O70348]<ref>PMID:10805743</ref> <ref>PMID:12612077</ref> <ref>PMID:12897126</ref> <ref>PMID:15565157</ref> <ref>PMID:16131592</ref> <ref>PMID:20802481</ref> <ref>PMID:26101253</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Synthetic construct]]
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[[Category: Zeng Y]]
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[[Category: Zhang Y]]

Current revision

Cryo-EM structure of yeast Rat1-bound Pol II pre-termination transcription complex 1 (Pol II Rat1-PTTC1)

PDB ID 8jch

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