5cvs

From Proteopedia

(Difference between revisions)

Current revision

GlgE isoform 1 from Streptomyces coelicolor E423A mutant soaked in maltoheptaose

Structural highlights

5cvs is a 2 chain structure with sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLGE1_STRCO Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP6 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Alpha-D-glucose 1-phosphate cannot serve as a donor substrate, but alpha-maltosyl fluoride is an efficient donor in vitro. Exhibits an alpha-retaining catalytic mechanism, with evidence that maltooligosaccharide acceptors are extended at their non-reducing ends. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen or maltoheptaose in the presence of inorganic phosphate. Also catalyzes disproportionation reactions through maltosyl transfer between maltooligosaccharides. Is probably involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.^[1]

Publication Abstract from PubMed

GlgE is a maltosyltranferase involved in alpha-glucan biosynthesis in bacteria that has been genetically validated as a target for tuberculosis therapies. Crystals of the Mycobacterium tuberculosis enzyme diffract at low resolution so most structural studies have been with the very similar Streptomyces coelicolor GlgE isoform 1. Although the donor binding site for alpha-1-phosphate had been previously structurally defined, the acceptor site had not. Using mutagenesis, kinetics and protein crystallography of the S. coelicolor enzyme, we have now identified the +1 to +6 sub-sites of the acceptor/product, which overlap with the known cyclodextrin binding site. The sugar residues in the acceptor sub-sites +1 to +5 are oriented such that they disfavour the binding of malto-oligosaccharides that bear branches at their 6 positions, consistent with the known acceptor chain specificity of GlgE. A secondary binding site remote from the catalytic centre was identified that is distinct from one reported for the M. tuberculosis enzyme. This new site is capable of binding a branched alpha-glucan, and is most likely involved in guiding acceptors towards the donor site because its disruption kinetically compromises the ability of GlgE to extend polymeric substrates. However, disruption of this site, which is conserved in the S. venezuelae GlgE enzyme, did not affect the growth of S. venezuelae or the structure of the polymeric product. The acceptor sub-sites +1 to +4 in the S. coelicolor enzyme are well conserved in the M. tuberculosis enzyme so their identification could help inform the design of inhibitors with therapeutic potential.

Ligand-bound structures and site-directed mutagenesis identify the acceptor and secondary binding sites of Streptomyces coelicolor maltosyltransferase GlgE.,Syson K, Stevenson CE, Miah F, Barclay JE, Tang M, Gorelik A, Rashid AM, Lawson DM, Bornemann S J Biol Chem. 2016 Aug 16. pii: jbc.M116.748160. PMID:27531751^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Syson K, Stevenson CE, Rejzek M, Fairhurst SA, Nair A, Bruton CJ, Field RA, Chater KF, Lawson DM, Bornemann S. Structure of a Streptomyces maltosyltransferase GlgE: a homologue of a genetically validated anti-tuberculosis target. J Biol Chem. 2011 Sep 13. PMID:21914799 doi:10.1074/jbc.M111.279315
↑ Syson K, Stevenson CE, Miah F, Barclay JE, Tang M, Gorelik A, Rashid AM, Lawson DM, Bornemann S. Ligand-bound structures and site-directed mutagenesis identify the acceptor and secondary binding sites of Streptomyces coelicolor maltosyltransferase GlgE. J Biol Chem. 2016 Aug 16. pii: jbc.M116.748160. PMID:27531751 doi:http://dx.doi.org/10.1074/jbc.M116.748160

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5cvs"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5cvs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CVS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900030:alpha-maltopentaose'>PRD_900030</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900030:alpha-maltopentaose'>PRD_900030</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cvs OCA], [https://pdbe.org/5cvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cvs RCSB], [https://www.ebi.ac.uk/pdbsum/5cvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cvs ProSAT]</span></td></tr>
 </table>

5cvs

From Proteopedia

Current revision

GlgE isoform 1 from Streptomyces coelicolor E423A mutant soaked in maltoheptaose

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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