8pmb
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Minimal I-motif comprising three cytosine-cytosine+ base-pairs== | |
| + | <StructureSection load='8pmb' size='340' side='right'caption='[[8pmb]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8pmb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PMB FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DNR:2-DEOXY-N3-PROTONATED+CYTIDINE-5-MONOPHOSPHATE'>DNR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pmb OCA], [https://pdbe.org/8pmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pmb RCSB], [https://www.ebi.ac.uk/pdbsum/8pmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pmb ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA sequences containing at least four runs of repetitive cytosines can fold into tetra-helical structures called i-Motifs (iMs). The interest in these DNA secondary structures is increasing due to their therapeutical and technological applications. Still, limited knowledge of their folding requirements is currently available. We developed a novel step-by-step pipeline for the systematic screening of putative iM-forming model sequences. Focusing on structures comprising only three cytosine-cytosine(+) base pairs, we investigated what the minimal lengths of the loops required for formation of an intra-molecular iM are. Our data indicate that two and three nucleotides are required to connect the strands through the minor and majorgrooves of the iM, respectively. Additionally, they highlight an asymmetric behavior according to the distribution of the cytosines. Specifically, no sequence containing a single cytosine in the first and third run was able to fold into intra-molecular iMs with the same stability of those formed when the first and the third run comprise two cytosines. This knowledge represents a step forward toward the development of prediction tools for the proper identification of biologically functional iMs, as well as for the rational design of these secondary structures as technological devices. | ||
| - | + | A Screening Protocol for Exploring Loop Length Requirements for the Formation of a Three Cytosine-Cytosine(+) Base-Paired i-Motif.,Ghezzo M, Trajkovski M, Plavec J, Sissi C Angew Chem Int Ed Engl. 2023 Oct 9;62(41):e202309327. doi: , 10.1002/anie.202309327. Epub 2023 Sep 4. PMID:37611164<ref>PMID:37611164</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8pmb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ghezzo M]] | ||
| + | [[Category: Plavec J]] | ||
| + | [[Category: Sissi C]] | ||
| + | [[Category: Trajkovski M]] | ||
Revision as of 08:30, 11 October 2023
Minimal I-motif comprising three cytosine-cytosine+ base-pairs
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