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1lf0

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{{STRUCTURE_1lf0| PDB=1lf0 | SCENE= }}
{{STRUCTURE_1lf0| PDB=1lf0 | SCENE= }}
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'''Crystal Structure of RasA59G in the GTP-bound form'''
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===Crystal Structure of RasA59G in the GTP-bound form===
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==Overview==
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The conformational changes in Ras that accompany the hydrolysis of GTP are critical to its function as a molecular switch in signaling pathways. Understanding how GTP is hydrolyzed by revealing the sequence of intermediary structures in the reaction is essential for understanding Ras signaling. Until now, no structure of an intermediate in GTP hydrolysis has been experimentally determined for Ras alone. We have solved the crystal structure of the Ala-59 to Gly mutant of Ras, (RasA59G), bound to guanosine 5'-imidotriphosphate or GDP to 1.7-A resolution. In the guanosine 5'-imidotriphosphate-bound form, this mutant adopts a conformation that is intermediate between the GTP- and GDP-bound forms of wild-type Ras and that is similar to what has been predicted by molecular dynamics simulation [Ma, J. P. &amp; Karplus, M. (1997) Proc. Natl. Acad. Sci. USA 94, 11905-11910]. This conformation is stabilized by direct and water-mediated interactions between the switch 1 and switch 2 regions and is characterized by an increase in the binding affinity for GTP. We propose that the structural changes promoted by the Ala-59 to Gly mutation exhibit a discrete conformational state assumed by wild-type Ras during GTP hydrolysis.
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The line below this paragraph, {{ABSTRACT_PUBMED_12213964}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12213964 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12213964}}
==About this Structure==
==About this Structure==
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[[Category: Intermediate]]
[[Category: Intermediate]]
[[Category: Ra]]
[[Category: Ra]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:51:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 17:07:06 2008''

Revision as of 14:20, 2 July 2008

Image:1lf0.png

Template:STRUCTURE 1lf0

Crystal Structure of RasA59G in the GTP-bound form

Template:ABSTRACT PUBMED 12213964

About this Structure

1LF0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The structural basis for the transition from Ras-GTP to Ras-GDP., Hall BE, Bar-Sagi D, Nassar N, Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12138-42. Epub 2002 Sep 4. PMID:12213964

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