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1lkb

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{{STRUCTURE_1lkb| PDB=1lkb | SCENE= }}
{{STRUCTURE_1lkb| PDB=1lkb | SCENE= }}
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'''Porcine Pancreatic Elastase/Na-Complex'''
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===Porcine Pancreatic Elastase/Na-Complex===
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==Overview==
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Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences.
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{{ABSTRACT_PUBMED_12198296}}
==About this Structure==
==About this Structure==
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[[Category: Sodium binding]]
[[Category: Sodium binding]]
[[Category: Sulfate binding]]
[[Category: Sulfate binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:17:31 2008''

Revision as of 18:17, 2 July 2008

Image:1lkb.png

Template:STRUCTURE 1lkb

Porcine Pancreatic Elastase/Na-Complex

Template:ABSTRACT PUBMED 12198296

About this Structure

1LKB is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Metal binding to porcine pancreatic elastase: calcium or not calcium., Weiss MS, Panjikar S, Nowak E, Tucker PA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1407-12. Epub 2002, Aug 23. PMID:12198296

Page seeded by OCA on Wed Jul 2 21:17:31 2008

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