8t7f

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'''Unreleased structure'''
 
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The entry 8t7f is ON HOLD until Paper Publication
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==Structure of the S1 variant of Fab F1==
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<StructureSection load='8t7f' size='340' side='right'caption='[[8t7f]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8t7f]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8T7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8T7F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8t7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8t7f OCA], [https://pdbe.org/8t7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8t7f RCSB], [https://www.ebi.ac.uk/pdbsum/8t7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8t7f ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The atomic-resolution structural information that X-ray crystallography can provide on the binding interface between a Fab and its cognate antigen is highly valuable for understanding the mechanism of interaction. However, many Fab:antigen complexes are recalcitrant to crystallization, making the endeavor a considerable effort with no guarantee of success. Consequently, there have been significant steps taken to increase the likelihood of Fab:antigen complex crystallization by altering the Fab framework. In this investigation, we applied the surface entropy reduction strategy coupled with phage-display technology to identify a set of surface substitutions that improve the propensity of a human Fab framework to crystallize. In addition, we showed that combining these surface substitutions with previously reported Crystal Kappa and elbow substitutions results in an extraordinary improvement in Fab and Fab:antigen complex crystallisability, revealing a strong synergistic relationship between these sets of substitutions. Through comprehensive Fab and Fab:antigen complex crystallization screenings followed by structure determination and analysis, we defined the roles that each of these substitutions play in facilitating crystallization and how they complement each other in the process. This article is protected by copyright. All rights reserved.
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Authors: Singer, A.U., Bruce, H.A., Enderle, L., Blazer, L., Adams, J.J., Sicheri, F., Sidhu, S.S.
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Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes.,Bruce HA, Singer AU, Filippova EV, Blazer LL, Adams JJ, Enderle L, Ben-David M, Radley EH, Mao DYL, Pau V, Orlicky S, Sicheri F, Kourinov I, Atwell S, Kossiakoff AA, Sidhu SS Protein Sci. 2023 Nov 9:e4824. doi: 10.1002/pro.4824. PMID:37945533<ref>PMID:37945533</ref>
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Description: Structure of the S1 variant of Fab F1
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Bruce, H.A]]
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<div class="pdbe-citations 8t7f" style="background-color:#fffaf0;"></div>
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[[Category: Singer, A.U]]
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== References ==
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[[Category: Sidhu, S.S]]
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<references/>
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[[Category: Adams, J.J]]
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__TOC__
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[[Category: Sicheri, F]]
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</StructureSection>
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[[Category: Enderle, L]]
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[[Category: Homo sapiens]]
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[[Category: Blazer, L]]
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[[Category: Large Structures]]
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[[Category: Adams JJ]]
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[[Category: Blazer L]]
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[[Category: Bruce HA]]
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[[Category: Enderle L]]
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[[Category: Sicheri F]]
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[[Category: Sidhu SS]]
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[[Category: Singer AU]]

Revision as of 07:33, 22 November 2023

Structure of the S1 variant of Fab F1

PDB ID 8t7f

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