1lf8
From Proteopedia
(New page: 200px<br /> <applet load="1lf8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lf8, resolution 2.30Å" /> '''Complex of GGA3-VHS...) |
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| - | [[Image:1lf8.gif|left|200px]]<br /> | + | [[Image:1lf8.gif|left|200px]]<br /><applet load="1lf8" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1lf8, resolution 2.30Å" /> | caption="1lf8, resolution 2.30Å" /> | ||
'''Complex of GGA3-VHS Domain and CI-MPR C-terminal Phosphopeptide'''<br /> | '''Complex of GGA3-VHS Domain and CI-MPR C-terminal Phosphopeptide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Phosphorylation of the cytosolic tails of transmembrane receptors can | + | Phosphorylation of the cytosolic tails of transmembrane receptors can regulate their intracellular trafficking. The structural basis for such regulation, however, has not been explained in most cases. The cytosolic tail of the cation-independent mannose 6-phosphate receptor contains a serine residue within an acidic-cluster dileucine signal that is important for the function of the receptor in the biosynthetic sorting of lysosomal hydrolases. We show here that phosphorylation of this Ser enhances interactions of the signal with its recognition module, the VHS domain of the GGA proteins. Crystallographic analyses demonstrate that the phosphoserine residue interacts electrostatically with two basic residues on the VHS domain of GGA3, thus providing an additional point of attachment of the acidic-cluster dileucine signal to its recognition module. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1LF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LF8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Bonifacino, J | + | [[Category: Bonifacino, J S.]] |
| - | [[Category: Hurley, J | + | [[Category: Hurley, J H.]] |
[[Category: Kato, Y.]] | [[Category: Kato, Y.]] | ||
[[Category: Misra, S.]] | [[Category: Misra, S.]] | ||
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[[Category: vhs domain]] | [[Category: vhs domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:44:26 2008'' |
Revision as of 11:44, 21 February 2008
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Complex of GGA3-VHS Domain and CI-MPR C-terminal Phosphopeptide
Contents |
Overview
Phosphorylation of the cytosolic tails of transmembrane receptors can regulate their intracellular trafficking. The structural basis for such regulation, however, has not been explained in most cases. The cytosolic tail of the cation-independent mannose 6-phosphate receptor contains a serine residue within an acidic-cluster dileucine signal that is important for the function of the receptor in the biosynthetic sorting of lysosomal hydrolases. We show here that phosphorylation of this Ser enhances interactions of the signal with its recognition module, the VHS domain of the GGA proteins. Crystallographic analyses demonstrate that the phosphoserine residue interacts electrostatically with two basic residues on the VHS domain of GGA3, thus providing an additional point of attachment of the acidic-cluster dileucine signal to its recognition module.
Disease
Known diseases associated with this structure: Hepatocellular carcinoma OMIM:[147280]
About this Structure
1LF8 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Phosphoregulation of sorting signal-VHS domain interactions by a direct electrostatic mechanism., Kato Y, Misra S, Puertollano R, Hurley JH, Bonifacino JS, Nat Struct Biol. 2002 Jul;9(7):532-6. PMID:12032548
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