This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1lj2
From Proteopedia
(New page: 200px<br /> <applet load="1lj2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lj2, resolution 2.38Å" /> '''Recognition of eIF4...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1lj2.gif|left|200px]]<br /> | + | [[Image:1lj2.gif|left|200px]]<br /><applet load="1lj2" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1lj2" size=" | + | |
caption="1lj2, resolution 2.38Å" /> | caption="1lj2, resolution 2.38Å" /> | ||
'''Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization'''<br /> | '''Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Rotaviruses, segmented double-stranded RNA viruses, co-opt the eukaryotic | + | Rotaviruses, segmented double-stranded RNA viruses, co-opt the eukaryotic translation machinery with the aid of nonstructural protein 3 (NSP3), a rotaviral functional homolog of the cellular poly(A) binding protein (PABP). NSP3 binds to viral mRNA 3' consensus sequences and circularizes mRNA via interactions with eIF4G. Here, we present the X-ray structure of the C-terminal domain of NSP3 (NSP3-C) recognizing a fragment of eIF4GI. Homodimerization of NSP3-C yields a symmetric, elongated, largely alpha-helical structure with two hydrophobic eIF4G binding pockets at the dimer interface. Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure. |
==About this Structure== | ==About this Structure== | ||
| - | 1LJ2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11] with AU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1LJ2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11] with <scene name='pdbligand=AU:'>AU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJ2 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 13: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Simian rotavirus a/sa11]] | [[Category: Simian rotavirus a/sa11]] | ||
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
| - | [[Category: Groft, C | + | [[Category: Groft, C M.]] |
[[Category: AU]] | [[Category: AU]] | ||
[[Category: nsp3; homodimer; eif4g; rotavirus; translation; mrna; closed loop; coiled coil]] | [[Category: nsp3; homodimer; eif4g; rotavirus; translation; mrna; closed loop; coiled coil]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:22 2008'' |
Revision as of 11:45, 21 February 2008
|
Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization
Overview
Rotaviruses, segmented double-stranded RNA viruses, co-opt the eukaryotic translation machinery with the aid of nonstructural protein 3 (NSP3), a rotaviral functional homolog of the cellular poly(A) binding protein (PABP). NSP3 binds to viral mRNA 3' consensus sequences and circularizes mRNA via interactions with eIF4G. Here, we present the X-ray structure of the C-terminal domain of NSP3 (NSP3-C) recognizing a fragment of eIF4GI. Homodimerization of NSP3-C yields a symmetric, elongated, largely alpha-helical structure with two hydrophobic eIF4G binding pockets at the dimer interface. Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure.
About this Structure
1LJ2 is a Protein complex structure of sequences from Simian rotavirus a/sa11 with as ligand. Full crystallographic information is available from OCA.
Reference
Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization., Groft CM, Burley SK, Mol Cell. 2002 Jun;9(6):1273-83. PMID:12086624
Page seeded by OCA on Thu Feb 21 13:45:22 2008
