1ljt
From Proteopedia
(New page: 200px<br /> <applet load="1ljt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ljt, resolution 2.Å" /> '''Crystal Structure of ...) |
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'''Crystal Structure of Macrophage Migration Inhibitory Factor complexed with (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole-acetic acid methyl ester (ISO-1)'''<br /> | '''Crystal Structure of Macrophage Migration Inhibitory Factor complexed with (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole-acetic acid methyl ester (ISO-1)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LJT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with HDI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1LJT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=HDI:'>HDI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-inhibitor complex]] | [[Category: protein-inhibitor complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:18:50 2008'' |
Revision as of 14:18, 15 February 2008
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Crystal Structure of Macrophage Migration Inhibitory Factor complexed with (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole-acetic acid methyl ester (ISO-1)
Contents |
Overview
Macrophage migration inhibitory factor (MIF) is an immunoregulatory, protein that is a potential therapeutic target for a number of, inflammatory diseases. Evidence exists that an unexpected catalytic active, site of MIF may have a biological function. To gain further insight into, the role of the catalytic active site, a series of mutational, structural, and biological activity studies were performed. The insertion of an, alanine between Pro-1 and Met-2 (PAM) abolishes a non-physiological, catalytic activity, and this mutant is defective in the in vitro, glucocorticoid counter-regulatory activity of MIF. The crystal structure, of MIF complexed to (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole, acetic acid methyl ester (ISO-1), an inhibitor of MIF d-dopachrome, tautomerase activity, reveals that ISO-1 binds to the same position of the, active site as p-hydroxyphenylpyruvic acid, a substrate of MIF. ISO-1, inhibits several MIF biological activities, further establishing a role, for the catalytic active site of MIF.
Disease
Known diseases associated with this structure: Persistent Mullerian duct syndrome, type I OMIM:[600957], Rheumatoid arthritis, systemic juvenile, susceptibility to OMIM:[153620]
About this Structure
1LJT is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
The tautomerase active site of macrophage migration inhibitory factor is a potential target for discovery of novel anti-inflammatory agents., Lubetsky JB, Dios A, Han J, Aljabari B, Ruzsicska B, Mitchell R, Lolis E, Al-Abed Y, J Biol Chem. 2002 Jul 12;277(28):24976-82. Epub 2002 May 7. PMID:11997397
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