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1lt9

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{{STRUCTURE_1lt9| PDB=1lt9 | SCENE= }}
{{STRUCTURE_1lt9| PDB=1lt9 | SCENE= }}
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'''Crystal Structure of Recombinant Human Fibrinogen Fragment D'''
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===Crystal Structure of Recombinant Human Fibrinogen Fragment D===
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==Overview==
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We report two crystal structures, each at a resolution of 2.8 A, of recombinant human fibrinogen fragment D (rfD) in the absence and presence of peptide ligands. The bound ligands, Gly-Pro-Arg-Pro-amide and Gly-His-Arg-Pro-amide, mimic the interactions of the thrombin exposed polymerization sites, "A" and "B", respectively. This report is the first to describe the structure of fragment D in the presence of both peptide ligands. The structures reveal that recombinant fibrinogen is nearly identical to the plasma protein but with minor changes, like the addition of a proximal fucose to the carbohydrate linked to residue betaGln364, and slightly different relative positions of the beta- and gamma-modules. Of major interest in our structures is that a previously identified calcium site in plasma fibrinogen is absent when Gly-His-Arg-Pro-amide is bound. The peptide-dependent loss of this calcium site may have significant biological implications that are further discussed. These structures provide a foundation for the detailed structural analysis of variant recombinant fibrinogens that were used to identify critical functional residues within fragment D.
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(as it appears on PubMed at http://www.pubmed.gov), where 12356313 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12356313}}
==About this Structure==
==About this Structure==
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[[Category: Recombinant fibrinogen]]
[[Category: Recombinant fibrinogen]]
[[Category: Recombinant fibrinogen fragment d]]
[[Category: Recombinant fibrinogen fragment d]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:15:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:09:19 2008''

Revision as of 19:09, 2 July 2008

Image:1lt9.png

Template:STRUCTURE 1lt9

Crystal Structure of Recombinant Human Fibrinogen Fragment D

Template:ABSTRACT PUBMED 12356313

About this Structure

1LT9 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

2.8 A crystal structures of recombinant fibrinogen fragment D with and without two peptide ligands: GHRP binding to the "b" site disrupts its nearby calcium-binding site., Kostelansky MS, Betts L, Gorkun OV, Lord ST, Biochemistry. 2002 Oct 8;41(40):12124-32. PMID:12356313

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