1lww
From Proteopedia
(New page: 200px<br /> <applet load="1lww" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lww, resolution 2.1Å" /> '''Borohydride-trapped ...) |
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| - | [[Image:1lww.gif|left|200px]]<br /> | + | [[Image:1lww.gif|left|200px]]<br /><applet load="1lww" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1lww" size=" | + | |
caption="1lww, resolution 2.1Å" /> | caption="1lww, resolution 2.1Å" /> | ||
'''Borohydride-trapped hOgg1 Intermediate Structure Co-Crystallized with 8-bromoguanine'''<br /> | '''Borohydride-trapped hOgg1 Intermediate Structure Co-Crystallized with 8-bromoguanine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Most spontaneous damage to bases in DNA is corrected through the action of | + | Most spontaneous damage to bases in DNA is corrected through the action of the base-excision DNA repair pathway. Base excision repair is initiated by DNA glycosylases, lesion-specific enzymes that intercept aberrant bases in DNA and catalyze their excision. How such proteins accomplish the feat of catalyzing no fewer than five sequential reaction steps using a single active site has been unknown. To help answer this, we report the structure of a trapped catalytic intermediate in DNA repair by human 8-oxoguanine DNA glycosylase. This structure and supporting biochemical results reveal that the enzyme sequesters the excised lesion base and exploits it as a cofactor to participate in catalysis. To our knowledge, the present example represents the first documented case of product-assisted catalysis in an enzyme-catalyzed reaction. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LWW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and BRG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1LWW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=BRG:'>BRG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bruner, S | + | [[Category: Bruner, S D.]] |
| - | [[Category: Fromme, J | + | [[Category: Fromme, J C.]] |
[[Category: Karplus, M.]] | [[Category: Karplus, M.]] | ||
| - | [[Category: Verdine, G | + | [[Category: Verdine, G L.]] |
[[Category: Yang, W.]] | [[Category: Yang, W.]] | ||
[[Category: BRG]] | [[Category: BRG]] | ||
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[[Category: reaction intermediate]] | [[Category: reaction intermediate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:15 2008'' |
Revision as of 11:49, 21 February 2008
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Borohydride-trapped hOgg1 Intermediate Structure Co-Crystallized with 8-bromoguanine
Contents |
Overview
Most spontaneous damage to bases in DNA is corrected through the action of the base-excision DNA repair pathway. Base excision repair is initiated by DNA glycosylases, lesion-specific enzymes that intercept aberrant bases in DNA and catalyze their excision. How such proteins accomplish the feat of catalyzing no fewer than five sequential reaction steps using a single active site has been unknown. To help answer this, we report the structure of a trapped catalytic intermediate in DNA repair by human 8-oxoguanine DNA glycosylase. This structure and supporting biochemical results reveal that the enzyme sequesters the excised lesion base and exploits it as a cofactor to participate in catalysis. To our knowledge, the present example represents the first documented case of product-assisted catalysis in an enzyme-catalyzed reaction.
Disease
Known disease associated with this structure: Renal cell carcinoma, clear cell, somatic OMIM:[601982]
About this Structure
1LWW is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Product-assisted catalysis in base-excision DNA repair., Fromme JC, Bruner SD, Yang W, Karplus M, Verdine GL, Nat Struct Biol. 2003 Mar;10(3):204-11. PMID:12592398
Page seeded by OCA on Thu Feb 21 13:49:15 2008
