8k8l
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Klebsiella pneumonia ModA with molybdate== | |
| + | <StructureSection load='8k8l' size='340' side='right'caption='[[8k8l]], [[Resolution|resolution]] 1.77Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8k8l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae_HS11286 Klebsiella pneumoniae subsp. pneumoniae HS11286]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8K8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8K8L FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8k8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8k8l OCA], [https://pdbe.org/8k8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8k8l RCSB], [https://www.ebi.ac.uk/pdbsum/8k8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8k8l ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0H3GU89_KLEPH A0A0H3GU89_KLEPH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Klebsiella pneumoniae, a facultative anaerobe, relies on acquiring molybdenum to sustain growth in anaerobic conditions, a crucial factor for the pathogen to establish infections within host environments. Molybdenum plays a critical role in pathogenesis as it forms an essential component of cofactors for molybdoenzymes. K. pneumoniae utilizes the ABC (ATP-Binding-Cassette) transporter encoded by the modABC operon for uptake of the group VI elements molybdenum and tungsten. In this study, we determined the X-ray crystal structures of both the molybdenum-free and molybdenum-bound substrate-binding protein (SBP) ModA from Klebsiella pneumoniae to 2.00 A and 1.77 A resolution respectively. ModA crystallizes in the space group P222 with a single monomer in one asymmetric unit. The purified protein remained soluble and specifically bound molybdate and tungstate with K(d) values of 6.3 nM and 5.2 nM, respectively. Tungstate competes with molybdate by binding to ModA, resulting in enhanced antimicrobial activity. These data provide a starting point for structural and functional analyses of molybdate transport in K. pneumoniae. | ||
| - | + | Structural analysis of molybdate binding protein ModA from Klebsiella pneumoniae.,Zhao Q, Su X, Wang Y, Liu R, Bartlam M Biochem Biophys Res Commun. 2023 Nov 12;681:41-46. doi: , 10.1016/j.bbrc.2023.09.055. Epub 2023 Sep 21. PMID:37751633<ref>PMID:37751633</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Bartlam | + | <div class="pdbe-citations 8k8l" style="background-color:#fffaf0;"></div> |
| - | [[Category: Zhao | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Klebsiella pneumoniae subsp. pneumoniae HS11286]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bartlam M]] | ||
| + | [[Category: Zhao Q]] | ||
Current revision
Structure of Klebsiella pneumonia ModA with molybdate
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