This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1lz9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1lz9.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1lz9.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1lz9| PDB=1lz9 | SCENE= }}
{{STRUCTURE_1lz9| PDB=1lz9 | SCENE= }}
-
'''ANOMALOUS SIGNAL OF SOLVENT BROMINES USED FOR PHASING OF LYSOZYME'''
+
===ANOMALOUS SIGNAL OF SOLVENT BROMINES USED FOR PHASING OF LYSOZYME===
-
==Overview==
+
<!--
-
The anomalous signal of bromide ions, present in the crystal structure of tetragonal hen egg-white lysozyme through the substitution of NaCl by NaBr in the crystallization medium, was used for phasing of X-ray data collected to 1.7 A resolution with a wavelength near the absorption edge of bromine. Phasing of a single wavelength data set, based purely on anomalous deltaf " contribution, led to easily interpretable electron density, equivalent to the complete multiwavelength anonalous dispersion phasing based on four-wavelength data. The classic small-structure direct methods program SHELXS run against all anomalous differences gave a successful solution of six highest peaks corresponding to six bromide ions in the structure with data limited up to a resolution of 3.5 A. Interpretable maps were obtained at a resolution up to 3.0 A using programs MLPHARE and DM. Bromide ions occupy well ordered positions at the protein surface. Phasing based on the single wavelength signal of anomalous scatterers introduced into the ordered solvent shell can be proposed as a tool for solving structures of well diffracting crystals.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10339408}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10339408 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10339408}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Single wavelength]]
[[Category: Single wavelength]]
[[Category: Solvent bromide]]
[[Category: Solvent bromide]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:26:42 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:52:06 2008''

Revision as of 19:52, 2 July 2008

Image:1lz9.png

Template:STRUCTURE 1lz9

ANOMALOUS SIGNAL OF SOLVENT BROMINES USED FOR PHASING OF LYSOZYME

Template:ABSTRACT PUBMED 10339408

About this Structure

1LZ9 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Anomalous signal of solvent bromides used for phasing of lysozyme., Dauter Z, Dauter M, J Mol Biol. 1999 May 28;289(1):93-101. PMID:10339408

Page seeded by OCA on Wed Jul 2 22:52:06 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1lz9"
Personal tools