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1m0d

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{{STRUCTURE_1m0d| PDB=1m0d | SCENE= }}
{{STRUCTURE_1m0d| PDB=1m0d | SCENE= }}
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'''Crystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active Site and Bound Manganese Ions'''
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===Crystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active Site and Bound Manganese Ions===
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==Overview==
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T7 endonuclease I is a nuclease that is selective for the structure of the four-way DNA junction. The active site is similar to those of a number of restriction enzymes. We have solved the crystal structure of endonuclease I with a wild-type active site. Diffusion of manganese ions into the crystal revealed two peaks of electron density per active site, defining two metal ion-binding sites. Site 1 is fully occupied, and the manganese ion is coordinated by the carboxylate groups of Asp55 and Glu65, and the main chain carbonyl of Thr66. Site 2 is partially occupied, and the metal ion has a single protein ligand, the remaining carboxylate oxygen atom of Asp55. Isothermal titration calorimetry showed the sequential exothermic binding of two manganese ions in solution, with dissociation constants of 0.58 +/- 0.019 and 14 +/- 1.5 mM. These results are consistent with a two metal ion mechanism for the cleavage reaction, in which the hydrolytic water molecule is contained in the first coordination sphere of the site 1-bound metal ion.
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(as it appears on PubMed at http://www.pubmed.gov), where 12093751 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12093751}}
==About this Structure==
==About this Structure==
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[[Category: Holliday junction resolvase]]
[[Category: Holliday junction resolvase]]
[[Category: Homodimer]]
[[Category: Homodimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:28:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:56:26 2008''

Revision as of 19:56, 2 July 2008

Image:1m0d.png

Template:STRUCTURE 1m0d

Crystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active Site and Bound Manganese Ions

Template:ABSTRACT PUBMED 12093751

About this Structure

1M0D is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.

Reference

Metal ions bound at the active site of the junction-resolving enzyme T7 endonuclease I., Hadden JM, Declais AC, Phillips SE, Lilley DM, EMBO J. 2002 Jul 1;21(13):3505-15. PMID:12093751

Page seeded by OCA on Wed Jul 2 22:56:26 2008

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