1m1a
From Proteopedia
(New page: 200px<br /> <applet load="1m1a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m1a, resolution 2.65Å" /> '''LIGAND BINDING ALTE...) |
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| - | [[Image:1m1a.gif|left|200px]]<br /> | + | [[Image:1m1a.gif|left|200px]]<br /><applet load="1m1a" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1m1a" size=" | + | |
caption="1m1a, resolution 2.65Å" /> | caption="1m1a, resolution 2.65Å" /> | ||
'''LIGAND BINDING ALTERS THE STRUCTURE AND DYNAMICS OF NUCLEOSOMAL DNA'''<br /> | '''LIGAND BINDING ALTERS THE STRUCTURE AND DYNAMICS OF NUCLEOSOMAL DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We determined the crystal structures of three nucleosome core particles in | + | We determined the crystal structures of three nucleosome core particles in complex with site-specific DNA-binding ligands, the pyrrole-imidazole polyamides. While the structure of the histone octamer and its interaction with the DNA remain unaffected by ligand binding, nucleosomal DNA undergoes significant structural changes at the ligand-binding sites and in adjacent regions to accommodate the ligands. Our findings suggest that twist diffusion occurs over long distances through tightly bound nucleosomal DNA. This may be relevant to the mechanism of ATP-dependent and spontaneous nucleosome translocation, and to the effect of bound factors on nucleosome dynamics. |
==About this Structure== | ==About this Structure== | ||
| - | 1M1A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with MN and IMT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1M1A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=IMT:'>IMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1A OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Xenopus laevis]] | [[Category: Xenopus laevis]] | ||
| - | [[Category: Dervan, P | + | [[Category: Dervan, P B.]] |
| - | [[Category: Edayathumangalam, R | + | [[Category: Edayathumangalam, R S.]] |
| - | [[Category: Gottesfeld, J | + | [[Category: Gottesfeld, J M.]] |
[[Category: Luger, K.]] | [[Category: Luger, K.]] | ||
[[Category: Melander, C.]] | [[Category: Melander, C.]] | ||
| - | [[Category: Suto, R | + | [[Category: Suto, R K.]] |
| - | [[Category: White, C | + | [[Category: White, C L.]] |
[[Category: IMT]] | [[Category: IMT]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: pyrrole-imidazole polyamide]] | [[Category: pyrrole-imidazole polyamide]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:50:26 2008'' |
Revision as of 11:50, 21 February 2008
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LIGAND BINDING ALTERS THE STRUCTURE AND DYNAMICS OF NUCLEOSOMAL DNA
Overview
We determined the crystal structures of three nucleosome core particles in complex with site-specific DNA-binding ligands, the pyrrole-imidazole polyamides. While the structure of the histone octamer and its interaction with the DNA remain unaffected by ligand binding, nucleosomal DNA undergoes significant structural changes at the ligand-binding sites and in adjacent regions to accommodate the ligands. Our findings suggest that twist diffusion occurs over long distances through tightly bound nucleosomal DNA. This may be relevant to the mechanism of ATP-dependent and spontaneous nucleosome translocation, and to the effect of bound factors on nucleosome dynamics.
About this Structure
1M1A is a Protein complex structure of sequences from Xenopus laevis with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structures of nucleosome core particles in complex with minor groove DNA-binding ligands., Suto RK, Edayathumangalam RS, White CL, Melander C, Gottesfeld JM, Dervan PB, Luger K, J Mol Biol. 2003 Feb 14;326(2):371-80. PMID:12559907
Page seeded by OCA on Thu Feb 21 13:50:26 2008
