1m3s

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{{STRUCTURE_1m3s| PDB=1m3s | SCENE= }}
{{STRUCTURE_1m3s| PDB=1m3s | SCENE= }}
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'''Crystal structure of YckF from Bacillus subtilis'''
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===Crystal structure of YckF from Bacillus subtilis===
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==Overview==
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The crystal structure of the YckF protein from Bacillus subtilis was determined with MAD phasing and refined at 1.95A resolution. YckF forms a tight tetramer both in crystals and in solution. Conservation of such oligomerization in other phosphate sugar isomerases indicates that the crystallographically observed tetramer is physiologically relevant. The structure of YckF was compared to with its ortholog from Methanococcus jannaschii, MJ1247. Both of these proteins have phosphate hexulose isomerase activity, although neither of the organisms can utilize methane or methanol as source of energy and/or carbon. Extensive sequence and structural similarities with MJ1247 and with the isomerase domain of glucosamine-6-phosphate synthase from Escherichia coli allowed us to group residues contributing to substrate binding or catalysis. Few notable differences among these structures suggest possible cooperativity of the four active sites of the tetramer. Phylogenetic relationships between obligatory and facultative methylotrophs along with B. subtilis and E. coli provide clues about the possible evolution of genes as they loose their physiological importance.
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(as it appears on PubMed at http://www.pubmed.gov), where 15363790 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15363790}}
==About this Structure==
==About this Structure==
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[[Category: Psi]]
[[Category: Psi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:35:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:08:58 2008''

Revision as of 20:08, 2 July 2008

Image:1m3s.png

Template:STRUCTURE 1m3s

Crystal structure of YckF from Bacillus subtilis

Template:ABSTRACT PUBMED 15363790

About this Structure

1M3S is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Crystal structure of Bacillus subtilis YckF: structural and functional evolution., Sanishvili R, Wu R, Kim DE, Watson JD, Collart F, Joachimiak A, J Struct Biol. 2004 Oct;148(1):98-109. PMID:15363790

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