1m4y

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{{STRUCTURE_1m4y| PDB=1m4y | SCENE= }}
{{STRUCTURE_1m4y| PDB=1m4y | SCENE= }}
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'''Crystal structure of HslV from Thermotoga maritima'''
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===Crystal structure of HslV from Thermotoga maritima===
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==Overview==
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Heat-shock locus VU (HslVU) is an ATP-dependent proteolytic system and a prokaryotic homolog of the proteasome. It consists of HslV, the protease, and HslU, the ATPase and chaperone. We have cloned, sequenced and expressed both protein components from the hyperthermophile Thermotoga maritima. T. maritima HslU hydrolyzes a variety of nucleotides in a temperature-dependent manner, with the optimum lying between 75 and 80 degrees C. It is also nucleotide-unspecific for activation of HslV against amidolytic and caseinolytic activity. The Escherichia coli and T. maritima HslU proteins mutually stimulate HslV proteins from both sources, suggesting a conserved activation mechanism. The crystal structure of T. maritima HslV was determined and refined to 2.1-A resolution. The structure of the dodecameric enzyme is well conserved compared to those from E. coli and Haemophilus influenzae. A comparison of known HslV structures confirms the presence of a cation-binding site, although its exact role in the proteolytic mechanism of HslV remains unclear. Amongst factors responsible for the thermostability of T. maritima HslV, extensive ionic interactions/salt-bridge networks, which occur specifically in the T. maritima enzyme in comparison to its mesophilic counterparts, seem to play an important role.
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(as it appears on PubMed at http://www.pubmed.gov), where 12646382 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12646382}}
==About this Structure==
==About this Structure==
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[[Category: Song, H K.]]
[[Category: Song, H K.]]
[[Category: N-terminal catalytic threonine residue]]
[[Category: N-terminal catalytic threonine residue]]
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Revision as of 20:13, 2 July 2008

Image:1m4y.png

Template:STRUCTURE 1m4y

Crystal structure of HslV from Thermotoga maritima

Template:ABSTRACT PUBMED 12646382

About this Structure

1M4Y is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

Isolation and characterization of the prokaryotic proteasome homolog HslVU (ClpQY) from Thermotoga maritima and the crystal structure of HslV., Song HK, Bochtler M, Azim MK, Hartmann C, Huber R, Ramachandran R, Biophys Chem. 2003;100(1-3):437-52. PMID:12646382

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