1m53

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{{STRUCTURE_1m53| PDB=1m53 | SCENE= }}
{{STRUCTURE_1m53| PDB=1m53 | SCENE= }}
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'''CRYSTAL STRUCTURE OF ISOMALTULOSE SYNTHASE (PALI) FROM KLEBSIELLA SP. LX3'''
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===CRYSTAL STRUCTURE OF ISOMALTULOSE SYNTHASE (PALI) FROM KLEBSIELLA SP. LX3===
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==Overview==
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Isomaltulose synthase from Klebsiella sp. LX3 (PalI, EC 5.4.99.11) catalyzes the isomerization of sucrose to produce isomaltulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) and trehalulose (alpha-D-glucosylpyranosyl-1,1-d-fructofuranose). The PalI structure, solved at 2.2-A resolution with an R-factor of 19.4% and Rfree of 24.2%, consists of three domains: an N-terminal catalytic (beta/alpha)8 domain, a subdomain between N beta 3 and N alpha 3, and a C-terminal domain having seven beta-strands. The active site architecture of PalI is identical to that of other glycoside hydrolase family 13 members, suggesting a similar mechanism in substrate binding and hydrolysis. However, a unique RLDRD motif in the proximity of the active site has been identified and shown biochemically to be responsible for sucrose isomerization. A two-step reaction mechanism for hydrolysis and isomerization, which occurs in the same pocket is proposed based on both the structural and biochemical data. Selected C-terminal truncations have been shown to reduce and even abolish the enzyme activity, consistent with the predicted role of the C-terminal residues in the maintenance of enzyme conformation and active site topology.
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==About this Structure==
==About this Structure==
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[[Category: Klebsiella sp. lx3]]
[[Category: Klebsiella sp. lx3]]
[[Category: Sucrose isomerization]]
[[Category: Sucrose isomerization]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:13:52 2008''

Revision as of 20:13, 2 July 2008

Image:1m53.png

Template:STRUCTURE 1m53

CRYSTAL STRUCTURE OF ISOMALTULOSE SYNTHASE (PALI) FROM KLEBSIELLA SP. LX3

Template:ABSTRACT PUBMED 12819210

About this Structure

1M53 is a Single protein structure of sequence from Klebsiella sp. lx3. Full crystallographic information is available from OCA.

Reference

Isomaltulose synthase (PalI) of Klebsiella sp. LX3. Crystal structure and implication of mechanism., Zhang D, Li N, Lok SM, Zhang LH, Swaminathan K, J Biol Chem. 2003 Sep 12;278(37):35428-34. Epub 2003 Jun 20. PMID:12819210

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