8k3g
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of non-specific phospholipase C RePLC (Rasamsonia emersonii)== | |
| + | <StructureSection load='8k3g' size='340' side='right'caption='[[8k3g]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8k3g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rasamsonia_emersonii_CBS_393.64 Rasamsonia emersonii CBS 393.64]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8K3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8K3G FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8k3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8k3g OCA], [https://pdbe.org/8k3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8k3g RCSB], [https://www.ebi.ac.uk/pdbsum/8k3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8k3g ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0F4Z128_TALEM A0A0F4Z128_TALEM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nonspecific phospholipase C (NPC) plays a pivotal role in hydrolyzing phospholipids, releasing diacylglycerol horizontal line an essential second messenger. Extensive research has elucidated the structure and function of bacterial and plant NPCs, but our understanding of their fungal counterparts remains limited. Here, we present the first crystal structure of a fungal NPC derived from Rasamsonia emersonii (RePLC), unraveling its distinguishable features divergent from other known phospholipase C. Remarkably, the structure of RePLC contains solely the phosphoesterase domain without the crucial C-terminal domain (CTD) found in plant NPCs, although CTD is important for their activity. Through a comparative analysis of structural features among NPCs from diverse species combined with structure-based mutation analyses and bioinformatics methods, we propose a potential molecular mechanism that may universally underlie the catalysis of phospholipid hydrolysis in fungal NPCs. Furthermore, our study sheds light on the captivating evolutionary trajectory of enzymes across diverse species. | ||
| - | + | Crystal Structure of Fungal Nonspecific Phospholipase C Unveils a Distinct Catalytic Mechanism.,Feng C, Fang H, Wang F, Chen W, Xia LC, Lan D, Wang Y J Agric Food Chem. 2023 Nov 1;71(43):16352-16361. doi: 10.1021/acs.jafc.3c05155. , Epub 2023 Oct 6. PMID:37800479<ref>PMID:37800479</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8k3g" style="background-color:#fffaf0;"></div> |
| - | [[Category: Feng | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Rasamsonia emersonii CBS 393 64]] | ||
| + | [[Category: Feng CH]] | ||
| + | [[Category: Wang YH]] | ||
Current revision
Crystal structure of non-specific phospholipase C RePLC (Rasamsonia emersonii)
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