1m5r

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[[Image:1m5r.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1m5r| PDB=1m5r | SCENE= }}
{{STRUCTURE_1m5r| PDB=1m5r | SCENE= }}
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'''Ternary complex of T4 phage BGT with UDP and a 13 mer DNA duplex'''
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===Ternary complex of T4 phage BGT with UDP and a 13 mer DNA duplex===
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==Overview==
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T4 phage beta-glucosyltransferase (BGT) modifies T4 DNA. We crystallized BGT with UDP-glucose and a 13mer DNA fragment containing an abasic site. We obtained two crystal structures of a ternary complex BGT-UDP-DNA at 1.8A and 2.5A resolution, one with a Tris molecule and the other with a metal ion at the active site. Both structures reveal a large distortion in the bound DNA. BGT flips the deoxyribose moiety at the abasic site to an extra-helical position and induces a 40 degrees bend in the DNA with a marked widening of the major groove. The Tris molecule mimics the glucose moiety in its transition state. The base-flipping mechanism, which has so far been observed only for glycosylases, methyltransferases and endonucleases, is now reported for a glucosyltransferase. BGT is unique in binding and inserting a loop into the DNA duplex through the major groove only. Furthermore, BGT compresses the backbone DNA one base further than the target base on the 3'-side.
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(as it appears on PubMed at http://www.pubmed.gov), where 12445783 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12445783}}
==About this Structure==
==About this Structure==
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[[Category: Base-flipping]]
[[Category: Base-flipping]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:40:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:16:17 2008''

Revision as of 20:16, 2 July 2008

Image:1m5r.png

Template:STRUCTURE 1m5r

Ternary complex of T4 phage BGT with UDP and a 13 mer DNA duplex

Template:ABSTRACT PUBMED 12445783

About this Structure

1M5R is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

A base-flipping mechanism for the T4 phage beta-glucosyltransferase and identification of a transition-state analog., Lariviere L, Morera S, J Mol Biol. 2002 Nov 29;324(3):483-90. PMID:12445783

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