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1m7x

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{{STRUCTURE_1m7x| PDB=1m7x | SCENE= }}
{{STRUCTURE_1m7x| PDB=1m7x | SCENE= }}
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'''The X-ray Crystallographic Structure of Branching Enzyme'''
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===The X-ray Crystallographic Structure of Branching Enzyme===
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==Overview==
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Branching enzyme catalyzes the formation of alpha-1,6 branch points in either glycogen or starch. We report the 2.3-A crystal structure of glycogen branching enzyme from Escherichia coli. The enzyme consists of three major domains, an NH(2)-terminal seven-stranded beta-sandwich domain, a COOH-terminal domain, and a central alpha/beta-barrel domain containing the enzyme active site. While the central domain is similar to that of all the other amylase family enzymes, branching enzyme shares the structure of all three domains only with isoamylase. Oligosaccharide binding was modeled for branching enzyme using the enzyme-oligosaccharide complex structures of various alpha-amylases and cyclodextrin glucanotransferase and residues were implicated in oligosaccharide binding. While most of the oligosaccharides modeled well in the branching enzyme structure, an approximate 50 degrees rotation between two of the glucose units was required to avoid steric clashes with Trp(298) of branching enzyme. A similar rotation was observed in the mammalian alpha-amylase structure caused by an equivalent tryptophan residue in this structure. It appears that there are two binding modes for oligosaccharides in these structures depending on the identity and location of this aromatic residue.
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{{ABSTRACT_PUBMED_12196524}}
==About this Structure==
==About this Structure==
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[[Category: Alpha/beta barrel]]
[[Category: Alpha/beta barrel]]
[[Category: Beta sandwich]]
[[Category: Beta sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:44:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:24:33 2008''

Revision as of 20:24, 2 July 2008

Image:1m7x.png


PDB ID 1m7x

Drag the structure with the mouse to rotate
1m7x, resolution 2.30Å ()
Activity: 1,4-alpha-glucan branching enzyme, with EC number 2.4.1.18
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



The X-ray Crystallographic Structure of Branching Enzyme

Template:ABSTRACT PUBMED 12196524

About this Structure

1M7X is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The X-ray crystallographic structure of Escherichia coli branching enzyme., Abad MC, Binderup K, Rios-Steiner J, Arni RK, Preiss J, Geiger JH, J Biol Chem. 2002 Nov 1;277(44):42164-70. Epub 2002 Aug 23. PMID:12196524

Page seeded by OCA on Wed Jul 2 23:24:33 2008

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