1m8x
From Proteopedia
(New page: 200px<br /> <applet load="1m8x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m8x, resolution 2.20Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1m8x.gif|left|200px]]<br /> | + | [[Image:1m8x.gif|left|200px]]<br /><applet load="1m8x" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1m8x" size=" | + | |
caption="1m8x, resolution 2.20Å" /> | caption="1m8x, resolution 2.20Å" /> | ||
'''CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE1-14 RNA'''<br /> | '''CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE1-14 RNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Puf proteins are developmental regulators that control mRNA stability and | + | Puf proteins are developmental regulators that control mRNA stability and translation by binding sequences in the 3' untranslated regions of their target mRNAs. We have determined the structure of the RNA binding domain of the human Puf protein, Pumilio1, bound to a high-affinity RNA ligand. The RNA binds the concave surface of the molecule, where each of the protein's eight repeats makes contacts with a different RNA base via three amino acid side chains at conserved positions. We have mutated these three side chains in one repeat, thereby altering the sequence specificity of Pumilio1. Thus, the high affinity and specificity of the PUM-HD for RNA is achieved using multiple copies of a simple repeated motif. |
==About this Structure== | ==About this Structure== | ||
| - | 1M8X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1M8X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hall, T | + | [[Category: Hall, T M.T.]] |
[[Category: McLachlan, J.]] | [[Category: McLachlan, J.]] | ||
[[Category: Wang, X.]] | [[Category: Wang, X.]] | ||
| - | [[Category: Zamore, P | + | [[Category: Zamore, P D.]] |
[[Category: nanos response element]] | [[Category: nanos response element]] | ||
[[Category: puf domain]] | [[Category: puf domain]] | ||
[[Category: pumilio-homology domain]] | [[Category: pumilio-homology domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:52:45 2008'' |
Revision as of 11:52, 21 February 2008
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CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE1-14 RNA
Overview
Puf proteins are developmental regulators that control mRNA stability and translation by binding sequences in the 3' untranslated regions of their target mRNAs. We have determined the structure of the RNA binding domain of the human Puf protein, Pumilio1, bound to a high-affinity RNA ligand. The RNA binds the concave surface of the molecule, where each of the protein's eight repeats makes contacts with a different RNA base via three amino acid side chains at conserved positions. We have mutated these three side chains in one repeat, thereby altering the sequence specificity of Pumilio1. Thus, the high affinity and specificity of the PUM-HD for RNA is achieved using multiple copies of a simple repeated motif.
About this Structure
1M8X is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Modular recognition of RNA by a human pumilio-homology domain., Wang X, McLachlan J, Zamore PD, Hall TM, Cell. 2002 Aug 23;110(4):501-12. PMID:12202039
Page seeded by OCA on Thu Feb 21 13:52:45 2008
