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1mep

From Proteopedia

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{{STRUCTURE_1mep| PDB=1mep | SCENE= }}
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'''Crystal Structure of Streptavidin Double Mutant S45A/D128A with Biotin: Cooperative Hydrogen-Bond Interactions in the Streptavidin-Biotin System.'''
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===Crystal Structure of Streptavidin Double Mutant S45A/D128A with Biotin: Cooperative Hydrogen-Bond Interactions in the Streptavidin-Biotin System.===
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==Overview==
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The thermodynamic and structural cooperativity between the Ser45- and D128-biotin hydrogen bonds was measured by calorimetric and X-ray crystallographic studies of the S45A/D128A double mutant of streptavidin. The double mutant exhibits a binding affinity approximately 2x10(7) times lower than that of wild-type streptavidin at 25 degrees C. The corresponding reduction in binding free energy (DeltaDeltaG) of 10.1 kcal/mol was nearly completely due to binding enthalpy losses at this temperature. The loss of binding affinity is 11-fold greater than that predicted by a linear combination of the single-mutant energetic perturbations (8.7 kcal/mol), indicating that these two mutations interact cooperatively. Crystallographic characterization of the double mutant and comparison with the two single mutant structures suggest that structural rearrangements at the S45 position, when the D128 carboxylate is removed, mask the true energetic contribution of the D128-biotin interaction. Taken together, the thermodynamic and structural analyses support the conclusion that the wild-type hydrogen bond between D128-OD and biotin-N2 is thermodynamically stronger than that between S45-OG and biotin-N1.
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(as it appears on PubMed at http://www.pubmed.gov), where 16452627 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16452627}}
==About this Structure==
==About this Structure==
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[[Category: Biotin-binding protein]]
[[Category: Biotin-binding protein]]
[[Category: Homotetramer]]
[[Category: Homotetramer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:56:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:48:31 2008''

Revision as of 20:48, 2 July 2008

Image:1mep.png

Template:STRUCTURE 1mep

Crystal Structure of Streptavidin Double Mutant S45A/D128A with Biotin: Cooperative Hydrogen-Bond Interactions in the Streptavidin-Biotin System.

Template:ABSTRACT PUBMED 16452627

About this Structure

1MEP is a Single protein structure of sequence from Streptomyces avidinii. Full crystallographic information is available from OCA.

Reference

Cooperative hydrogen bond interactions in the streptavidin-biotin system., Hyre DE, Le Trong I, Merritt EA, Eccleston JF, Green NM, Stenkamp RE, Stayton PS, Protein Sci. 2006 Mar;15(3):459-67. Epub 2006 Feb 1. PMID:16452627

Page seeded by OCA on Wed Jul 2 23:48:31 2008

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