1mfa

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{{STRUCTURE_1mfa| PDB=1mfa | SCENE= }}
{{STRUCTURE_1mfa| PDB=1mfa | SCENE= }}
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'''STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION'''
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===STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION===
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==Overview==
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We describe here the 1.7-A resolution structure of a single-chain antibody variable domain (scFv) molecule, based on the carbohydrate-binding antibody Se155-4, complexed with the trisaccharide ligand alpha-D-Gal(1--&gt;2)[alpha-D-Abe(1--&gt;3)]alpha-D-Manp1--&gt;OMe, where Abe is abequose. The scFv expressed in Escherichia coli has the variable region light chain to heavy chain polarity with the domains connected by a 19-residue linker. Although the linker is partially disordered in the crystal, the packing of the molecules suggests a monomeric state of the scFv. The carbohydrate adopts a different conformation about the Man-Gal linkage than was observed previously in the Fab-trisaccharide complex. Instead of a direct hydrogen bond between O2Abe and O2Gal, these two atoms are bridged by a water molecule in the present complex.
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{{ABSTRACT_PUBMED_7517550}}
==About this Structure==
==About this Structure==
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[[Category: Zdanov, A.]]
[[Category: Zdanov, A.]]
[[Category: Immunoglobulin]]
[[Category: Immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:57:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:50:34 2008''

Revision as of 20:50, 2 July 2008

Image:1mfa.png

Template:STRUCTURE 1mfa

STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION

Template:ABSTRACT PUBMED 7517550

About this Structure

Full crystallographic information is available from OCA.

Reference

Structure of a single-chain antibody variable domain (Fv) fragment complexed with a carbohydrate antigen at 1.7-A resolution., Zdanov A, Li Y, Bundle DR, Deng SJ, MacKenzie CR, Narang SA, Young NM, Cygler M, Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6423-7. PMID:7517550

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